Genome-wide identification and data mining reveals major-latex protein (MLP) from the PR-10 protein family played defense-related roles against phytopathogenic challenges in cassava (Manihot esculenta Crantz).

IF 1.3 4区 生物学 Q4 GENETICS & HEREDITY
Genetica Pub Date : 2024-12-01 Epub Date: 2024-08-31 DOI:10.1007/s10709-024-00211-6
Unchera Viboonjun, Rawit Longsaward
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引用次数: 0

Abstract

Despite being identified in previous articles, the pathogenesis-related 10 (PR-10) protein remains relatively overlooked and has yet to be fully characterized in numerous plant species. This research employs a comprehensive data mining approach to in silico characterize PR-10 proteins in cassava, a vital crop plant globally. In this study, the focus was on in silico identified 53 cassava PR-10 proteins, which can be categorized into two main subgroups: 34 major latex proteins (MLPs) and 13 major allergen proteins, Pru ar 1, based on their phylogenetic relationship. The genome collinearity analysis with the rubber tree showed a possible evolutionary relationship of the PR-10 gene between these two Euphorbiaceae species, specifically on their chromosome 15. Notably, MLP423 and other MLP proteins were identified in various previously published cassava transcriptome datasets in response to biotic treatments from diverse phytopathogens, including anthracnose fungus, viruses, and bacterial blight. Ligand prediction and molecular docking of three MLP423 proteins have revealed potential interaction with cytokinin and abscisic acid hormones. Their expressions and predicted binding affinities are discussed here, highlighting their role as contributors to cassava's defense network against key diseases.

Abstract Image

通过全基因组鉴定和数据挖掘发现,PR-10 蛋白家族中的主要乳胶蛋白(MLP)在木薯(Manihot esculenta Crantz)的植物病原挑战中发挥着防御作用。
尽管在以前的文章中已经发现了病原相关 10(PR-10)蛋白,但它仍然相对被忽视,在许多植物物种中尚未得到全面表征。本研究采用了一种全面的数据挖掘方法,对全球重要的农作物--木薯中的 PR-10 蛋白进行了硅学表征。在这项研究中,重点研究了在硅学中鉴定出的 53 种木薯 PR-10 蛋白,根据它们之间的系统发育关系,这些蛋白可分为两大类:34 种主要乳胶蛋白(MLPs)和 13 种主要过敏原蛋白 Pru ar 1。与橡胶树的基因组共线性分析表明,这两种大戟科植物之间的 PR-10 基因可能存在进化关系,特别是在它们的 15 号染色体上。值得注意的是,在以前发表的各种木薯转录组数据集中,发现了 MLP423 和其他 MLP 蛋白对炭疽病菌、病毒和细菌性枯萎病等多种植物病原体的生物处理的响应。三个 MLP423 蛋白的配体预测和分子对接揭示了它们与细胞分裂素和脱落酸激素的潜在相互作用。本文讨论了它们的表达和预测的结合亲和力,强调了它们在木薯防御关键病害网络中的作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Genetica
Genetica 生物-遗传学
CiteScore
2.70
自引率
0.00%
发文量
32
审稿时长
>12 weeks
期刊介绍: Genetica publishes papers dealing with genetics, genomics, and evolution. Our journal covers novel advances in the fields of genomics, conservation genetics, genotype-phenotype interactions, evo-devo, population and quantitative genetics, and biodiversity. Genetica publishes original research articles addressing novel conceptual, experimental, and theoretical issues in these areas, whatever the taxon considered. Biomedical papers and papers on breeding animal and plant genetics are not within the scope of Genetica, unless framed in an evolutionary context. Recent advances in genetics, genomics and evolution are also published in thematic issues and synthesis papers published by experts in the field.
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