Oak seedling microbiome assembly under climate warming and drought.

IF 6.2 2区 环境科学与生态学 Q1 GENETICS & HEREDITY
Daniel Hoefle, Milena Sommer, Birgit Wassermann, Maria Faticov, Demetrio Serra, Gabriele Berg, Ayco J M Tack, Ahmed Abdelfattah
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Abstract

Despite that climate change is currently one of the most pervasive challenges, its effects on the plant-associated microbiome is still poorly studied. The aim of this study was to evaluate the impact of the independent and combinatory effect of climate warming and drought on the microbiome assembly of oak from seed to seedling. In a multifactorial experimental set up, acorns were subjected to different temperatures (15 °C, 20 °C, and 25 °C) and soil moisture levels (drought (15%) and control (60%)) from germination until the seedling stage, after which the bacterial and fungal communities associated to the rhizosphere and phyllosphere were characterized by amplicon sequencing and qPCR. The results showed a stronger effect of temperature on fungal than on bacterial diversity and the effect was more pronounced in the phyllosphere. Under drought condition, temperature had a significantly negative effect on phyllosphere fungal diversity. In the rhizosphere, temperature had a significant effect on the fungal community composition which was primarily caused by species turnover. Regardless of temperature, Actinobacteriota was significantly enriched in drought, a group of bacteria known to increase plant drought tolerance. This study provides new insights into the effect of climate change on the plant microbiome in natural ecosystems.

气候变暖和干旱条件下橡树幼苗微生物组的组合。
尽管气候变化是当前最普遍的挑战之一,但对其对植物相关微生物组的影响的研究仍然很少。本研究的目的是评估气候变暖和干旱对橡树从种子到幼苗的微生物组组合的独立和综合影响。在一个多因素实验装置中,橡子从萌芽到幼苗阶段分别受到不同温度(15 °C、20 °C和25 °C)和土壤水分水平(干旱(15%)和对照(60%))的影响,之后通过扩增子测序和 qPCR 鉴定了与根瘤层和叶球层相关的细菌和真菌群落。结果表明,温度对真菌多样性的影响强于对细菌多样性的影响,而且这种影响在植被层中更为明显。在干旱条件下,温度对叶球真菌多样性有明显的负面影响。在根瘤层,温度对真菌群落组成有显著影响,这主要是由于物种更替造成的。无论温度如何,放线菌群在干旱时明显富集,而放线菌群是已知能提高植物耐旱性的细菌群。这项研究为了解气候变化对自然生态系统中植物微生物群的影响提供了新的视角。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Environmental Microbiome
Environmental Microbiome Immunology and Microbiology-Microbiology
CiteScore
7.40
自引率
2.50%
发文量
55
审稿时长
13 weeks
期刊介绍: Microorganisms, omnipresent across Earth's diverse environments, play a crucial role in adapting to external changes, influencing Earth's systems and cycles, and contributing significantly to agricultural practices. Through applied microbiology, they offer solutions to various everyday needs. Environmental Microbiome recognizes the universal presence and significance of microorganisms, inviting submissions that explore the diverse facets of environmental and applied microbiological research.
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