Helicobacter pylori Infection Affects the Tumor Immune Microenvironment of Esophageal Cancer Patients.

IF 1.6 4区医学 Q4 ONCOLOGY

Anticancer research Pub Date : 2024-09-01 DOI:10.21873/anticanres.17205

Hiroki Matsuda, Kota Iwahori, Tomohira Takeoka, Ryo Kato, Shinya Urakawa, Takuro Saito, Tomoki Makino, Hidetoshi Eguchi, Yuichiro Doki, Hisashi Wada

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引用次数: 0

Abstract

Background/aim: We herein examined T cell immunity in esophageal cancer patients with and without Helicobacter pylori infection to establish a foundation for immunotherapeutic strategies targeting esophageal cancer in the presence of H. pylori infection.

Materials and methods: Twenty-six patients with esophageal squamous cell carcinoma between 2015 and 2017 were enrolled in the present study. Serum antibodies against H. pylori were measured. Fresh tumor tissues were obtained by endoscopic biopsy or from surgical resection. A cell suspension of these tissues was subjected to a flow cytometric analysis.

Results: Among the 26 patients analyzed, 10 (38.5%) were seropositive for H. pylori. The flow cytometric analysis of tumor-infiltrating lymphocytes revealed that the percentage of CD103⁺CD4⁺ T cells in esophageal tumors was significantly lower in H. pylori-positive patients than in H. pylori-negative patients (p=0.0105). Conversely, the percentage of CD45RA-CD25hi effector Treg cells in esophageal tumors was significantly higher in H. pylori-positive patients than in H. pylori-negative patients (p=0.0022), indicating an immunosuppressive tumor microenvironment in the former. Following neoadjuvant chemotherapy, the number of CD45RA-CD25hi effector Treg cells decreased (p=0.0248).

Conclusion: The tumor immune microenvironment of esophageal cancer patients with H. pylori infection exhibited an immunosuppressive phenotype. The targeting of Treg cells has potential in immunotherapy for this patient population.

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幽门螺杆菌感染影响食管癌患者的肿瘤免疫微环境

背景/目的：我们在此研究了幽门螺杆菌感染和未感染幽门螺杆菌的食管癌患者的T细胞免疫，为幽门螺杆菌感染情况下针对食管癌的免疫治疗策略奠定基础：本研究共纳入了26例2015年至2017年间的食管鳞状细胞癌患者。测定了血清中的幽门螺杆菌抗体。通过内镜活检或手术切除获得新鲜肿瘤组织。对这些组织的细胞悬液进行流式细胞分析：在分析的 26 名患者中，10 人（38.5%）幽门螺杆菌血清反应呈阳性。肿瘤浸润淋巴细胞的流式细胞分析显示，幽门螺杆菌阳性患者食管肿瘤中 CD103+CD4+ T 细胞的比例明显低于幽门螺杆菌阴性患者（P=0.0105）。相反，幽门螺杆菌阳性患者食管肿瘤中CD45RA-CD25hi效应Treg细胞的比例明显高于幽门螺杆菌阴性患者（p=0.0022），表明前者存在免疫抑制性肿瘤微环境。新辅助化疗后，CD45RA-CD25hi效应Treg细胞数量减少（p=0.0248）：结论：幽门螺杆菌感染的食管癌患者的肿瘤免疫微环境表现出免疫抑制表型。以Treg细胞为靶点的免疫疗法在这类患者中具有潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Anticancer research 医学-肿瘤学

CiteScore

3.70

自引率

10.00%

发文量

566

审稿时长

2 months

期刊介绍： ANTICANCER RESEARCH is an independent international peer-reviewed journal devoted to the rapid publication of high quality original articles and reviews on all aspects of experimental and clinical oncology. Prompt evaluation of all submitted articles in confidence and rapid publication within 1-2 months of acceptance are guaranteed. ANTICANCER RESEARCH was established in 1981 and is published monthly (bimonthly until the end of 2008). Each annual volume contains twelve issues and index. Each issue may be divided into three parts (A: Reviews, B: Experimental studies, and C: Clinical and Epidemiological studies). Special issues, presenting the proceedings of meetings or groups of papers on topics of significant progress, will also be included in each volume. There is no limitation to the number of pages per issue.