A new enzymatic clarification method for pomegranate juice: Removal of defects and improvement of quality by tannase, lactonase and papain

IF 3.5 2区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
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引用次数: 0

Abstract

Effects of clarification with “tannase,” “tannase+lactonase,” “papain,” “aspartic protease” and “tannase+lactonase+papain” on defects (turbidity, sediment and color loss) and important constituents (phenolics, anthocyanins, proteins and amino acids) of pomegranate juice (PJ) were investigated. Lactonase [human-derived (H-PON-1 and H-PON-2) and rabbit-derived (R-PON-1, R-PON-2 and R-PON-3)] was used during juice clarification for the first time. Phenolics and amino acids were quantified by HPLC-DAD-MS and HPLC. After clarifications with “tannase (1 g/L)+R-PON-1 (0.33 mg/L)” and “tannase (1 g/L)+R-PON-1 (0.33 mg/L)+papain (10 mg/L)” at 30℃ for 90 min, sediment was completely eliminated during storage at 20℃ for a month. Among these treatments, “tannase+R-PON-1” provided the lowest turbidity formation rate. The most effective parameter to prevent sediment was the ratio of “prolamin to trigalloyl-HHDP-glucose-isomer-2” that should be ≥205.4 (r=–1.000). Moreover, “tannase+R-PON-1” caused 4.3 times lower turbidity than control group after storage. To retain low turbidity (≤10 NTU) during storage, galloyl-glucose-isomer and HHDP-galloyl-hexoside-3 should completely be removed from PJ and, punigluconin and trigalloyl-HHDP-glucose-isomer-2 contents should be ≤0.19 and ≤0.04 mg/L, respectively. Furthermore, “tannase+R-PON-1” provided a significant contribution to anthocyanin copigmentation. The most effective copigments in PJ were HHDP-galloyl-glucuronide, punigluconin, punicalagin-2, punicalagin-3, aspartic acid, glutamic acid and alanine. “Tannase+R-PON-1” is recommended to produce a high-quality PJ.

石榴汁酶解澄清新方法:用单宁酶、乳蛋白酶和木瓜蛋白酶去除缺陷和提高质量
研究了使用 "单宁酸酶"、"单宁酸酶+乳酸菌素酶"、"木瓜蛋白酶"、"天冬氨酸蛋白酶 "和 "单宁酸酶+乳酸菌素酶+木瓜蛋白酶 "进行澄清对石榴汁(PJ)的缺陷(浑浊度、沉淀物和颜色损失)和重要成分(酚类、花青素、蛋白质和氨基酸)的影响。在果汁澄清过程中首次使用了乳酸菌酶[人源型(H-PON-1 和 H-PON-2)和兔源型(R-PON-1、R-PON-2 和 R-PON-3)]。酚类物质和氨基酸通过 HPLC-DAD-MS 和 HPLC 进行定量。用 "单宁酸酶(1 g/L)+R-PON-1(0.33 mg/L)"和 "单宁酸酶(1 g/L)+R-PON-1(0.33 mg/L)+木瓜蛋白酶(10 mg/L)"在 30℃ 下澄清 90 分钟后,在 20℃ 下贮藏一个月后,沉淀物完全消除。在这些处理中,"单宁酶+R-PON-1 "的浊度形成率最低。防止沉淀物产生的最有效参数是 "丙烯胺与三缩水甘油醚-葡萄糖异构体-2 "的比率,应≥205.4(r=-1.000)。此外,"鞣酸酶+R-PON-1 "贮藏后的浊度比对照组低 4.3 倍。要在贮藏期间保持低浊度(≤10 NTU),应完全去除 PJ 中的五倍子-葡萄糖异构体和 HHDP-五倍子-己糖苷-3,并且普尼格葡糖酸和三倍子-HHDP-葡萄糖异构体-2 的含量应分别≤0.19 和≤0.04 mg/L。此外,"单宁酶+R-PON-1 "对花青素共着色也有重要贡献。PJ 中最有效的花青素共聚物是 HHDP-galloyl-glucuronide、punigluconin、punicalagin-2、punicalagin-3、天冬氨酸、谷氨酸和丙氨酸。建议使用 "单宁酶+R-PON-1 "来生产高质量的 PJ。
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来源期刊
Food and Bioproducts Processing
Food and Bioproducts Processing 工程技术-工程:化工
CiteScore
9.70
自引率
4.30%
发文量
115
审稿时长
24 days
期刊介绍: Official Journal of the European Federation of Chemical Engineering: Part C FBP aims to be the principal international journal for publication of high quality, original papers in the branches of engineering and science dedicated to the safe processing of biological products. It is the only journal to exploit the synergy between biotechnology, bioprocessing and food engineering. Papers showing how research results can be used in engineering design, and accounts of experimental or theoretical research work bringing new perspectives to established principles, highlighting unsolved problems or indicating directions for future research, are particularly welcome. Contributions that deal with new developments in equipment or processes and that can be given quantitative expression are encouraged. The journal is especially interested in papers that extend the boundaries of food and bioproducts processing. The journal has a strong emphasis on the interface between engineering and food or bioproducts. Papers that are not likely to be published are those: • Primarily concerned with food formulation • That use experimental design techniques to obtain response surfaces but gain little insight from them • That are empirical and ignore established mechanistic models, e.g., empirical drying curves • That are primarily concerned about sensory evaluation and colour • Concern the extraction, encapsulation and/or antioxidant activity of a specific biological material without providing insight that could be applied to a similar but different material, • Containing only chemical analyses of biological materials.
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