Pathogen-driven Pseudomonas reshaped the phyllosphere microbiome in combination with Pseudostellaria heterophylla foliar disease resistance via the release of volatile organic compounds.

IF 6.2 2区 环境科学与生态学 Q1 GENETICS & HEREDITY
Qing-Song Yuan, Yanping Gao, Lu Wang, Xiaoai Wang, Lingling Wang, Jiayue Ran, Xiaohong Ou, Yanhong Wang, Chenghong Xiao, Weike Jiang, Lanping Guo, Tao Zhou, Luqi Huang
{"title":"Pathogen-driven Pseudomonas reshaped the phyllosphere microbiome in combination with Pseudostellaria heterophylla foliar disease resistance via the release of volatile organic compounds.","authors":"Qing-Song Yuan, Yanping Gao, Lu Wang, Xiaoai Wang, Lingling Wang, Jiayue Ran, Xiaohong Ou, Yanhong Wang, Chenghong Xiao, Weike Jiang, Lanping Guo, Tao Zhou, Luqi Huang","doi":"10.1186/s40793-024-00603-3","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Continuous monocropping obstacles are common in plants, especially medicinal plants, resulting in disease outbreaks and productivity reductions. Foliar disease, mainly caused by Fusarium oxysporum, results in a severe decrease in the yield of Pseudostellaria heterophylla annually. Determining an effective biomethod to alleviate this disease is urgently needed to improve its productivity and quality.</p><p><strong>Results: </strong>This study screened thirty-two keystone bacterial genera induced by pathogens in P. heterophylla rhizosphere soil under continuous monocropping conditions. Pseudomonas, Chryseobacterium, and Flavobacterium, referred to as the beneficial microbiota, were significantly attracted by pathogen infection. The P. palleroniana strain B-BH16-1 can directly inhibit the growth and spore formation of seven primary pathogens of P. heterophylla foliar disease by disrupting fusaric acid production via the emission of volatile organic compounds (VOCs). In addition, strain B-BH16-1 enhances the disease resistance of P. heterophylla by obliterating the pathogen and assembling beneficial microbiota.</p><p><strong>Conclusion: </strong>Pathogen-induced Pseudomonas reshaped phyllosphere microbial communities via direct antagonism of pathogens and indirect disruption of the pathogen virulence factor biosynthesis to enhance disease suppression and improve yields. These results show that inhibiting pathogen virulence biosynthesis to reshape the plant microbial community using disease-induing probiotics will be an innovative strategy for managing plant disease, especially under continuous monoculture conditions.</p>","PeriodicalId":48553,"journal":{"name":"Environmental Microbiome","volume":null,"pages":null},"PeriodicalIF":6.2000,"publicationDate":"2024-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11344943/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Environmental Microbiome","FirstCategoryId":"93","ListUrlMain":"https://doi.org/10.1186/s40793-024-00603-3","RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}
引用次数: 0

Abstract

Background: Continuous monocropping obstacles are common in plants, especially medicinal plants, resulting in disease outbreaks and productivity reductions. Foliar disease, mainly caused by Fusarium oxysporum, results in a severe decrease in the yield of Pseudostellaria heterophylla annually. Determining an effective biomethod to alleviate this disease is urgently needed to improve its productivity and quality.

Results: This study screened thirty-two keystone bacterial genera induced by pathogens in P. heterophylla rhizosphere soil under continuous monocropping conditions. Pseudomonas, Chryseobacterium, and Flavobacterium, referred to as the beneficial microbiota, were significantly attracted by pathogen infection. The P. palleroniana strain B-BH16-1 can directly inhibit the growth and spore formation of seven primary pathogens of P. heterophylla foliar disease by disrupting fusaric acid production via the emission of volatile organic compounds (VOCs). In addition, strain B-BH16-1 enhances the disease resistance of P. heterophylla by obliterating the pathogen and assembling beneficial microbiota.

Conclusion: Pathogen-induced Pseudomonas reshaped phyllosphere microbial communities via direct antagonism of pathogens and indirect disruption of the pathogen virulence factor biosynthesis to enhance disease suppression and improve yields. These results show that inhibiting pathogen virulence biosynthesis to reshape the plant microbial community using disease-induing probiotics will be an innovative strategy for managing plant disease, especially under continuous monoculture conditions.

病原体驱动的假单胞菌通过释放挥发性有机化合物重塑了叶球微生物组,并与异叶假叶藻叶片抗病性相结合。
背景:植物(尤其是药用植物)普遍存在连续单一种植的障碍,导致病害爆发和产量下降。主要由镰孢菌引起的叶面病害导致异叶假山每年严重减产。为提高其产量和质量,迫切需要确定一种有效的生物方法来缓解这种病害:结果:本研究筛选了 32 个由病原体诱导的关键细菌菌属,这些菌属存在于连续单作条件下的异叶假叶芹根瘤土壤中。被称为有益微生物群的假单胞菌、绿脓杆菌和黄杆菌受到病原体感染的显著吸引。P. palleroniana菌株B-BH16-1能通过释放挥发性有机化合物(VOCs)干扰镰刀菌酸的产生,从而直接抑制异型叶枯病七种主要病原体的生长和孢子形成。此外,菌株 B-BH16-1 还能通过抹杀病原体和聚集有益微生物群来增强异型叶枯病的抗病性:病原体诱导的假单胞菌通过直接拮抗病原体和间接破坏病原体毒力因子的生物合成来重塑叶球微生物群落,从而增强病害抑制能力并提高产量。这些结果表明,利用病原诱导型益生菌抑制病原毒力生物合成以重塑植物微生物群落,将是管理植物病害的一种创新策略,尤其是在连续单一栽培条件下。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Environmental Microbiome
Environmental Microbiome Immunology and Microbiology-Microbiology
CiteScore
7.40
自引率
2.50%
发文量
55
审稿时长
13 weeks
期刊介绍: Microorganisms, omnipresent across Earth's diverse environments, play a crucial role in adapting to external changes, influencing Earth's systems and cycles, and contributing significantly to agricultural practices. Through applied microbiology, they offer solutions to various everyday needs. Environmental Microbiome recognizes the universal presence and significance of microorganisms, inviting submissions that explore the diverse facets of environmental and applied microbiological research.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信