Investigating the Prognostic and Oncogenic Roles of Membrane-Associated Ring-CH-Type Finger 9 in Colorectal Cancer.

IF 1.4 4区 生物学 Q4 GENETICS & HEREDITY
Genetics research Pub Date : 2024-08-17 eCollection Date: 2024-01-01 DOI:10.1155/2024/9279653
Jiayan Zhang, Qinghan Jiao, Zhigang Chen
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Abstract

Backgroundsand Aims. Colorectal cancer (CRC) represents a major global health challenge, necessitating comprehensive investigations into its underlying molecular mechanisms to enhance diagnostic and therapeutic strategies. This study focuses on elucidating the oncogenic role of Membrane-Associated Ring-CH-Type Finger 9 (MARCHF9), a RING-Type E3 ubiquitin transferase, in CRC. We aim to assess MARCHF9's clinical significance, functional impact on CRC progression, and its potential as a prognostic biomarker. Methods. We leveraged data from the Cancer Genome Atlas (TCGA) cohort to evaluate MARCHF9 expression profiles in CRC. In vitro experiments involved siRNA-mediated MARCHF9 knockdown in COAD cell lines (SW480 and LoVo). Cell proliferation and invasion assays were conducted to investigate MARCHF9's functional relevance. Survival analyses were performed to assess its prognostic role. Results. Our analysis revealed significantly elevated MARCHF9 expression in CRC tissues compared to normal colorectal tissues (P < 0.05). High MARCHF9 expression correlated with advanced clinical stages, distant metastases, and the presence of residual tumors in CRC patients. Survival analyses demonstrated that high MARCHF9 expression predicted unfavorable overall and disease-free survival outcomes (P < 0.05). In vitro experiments further supported its oncogenic potential, with MARCHF9 knockdown inhibiting COAD cell proliferation and invasion. Conclusions. This study unveils the oncogenic role of MARCHF9 in CRC, highlighting its clinical relevance as a potential biomarker and therapeutic target. MARCHF9's association with adverse clinicopathological features and its functional impact on cancer cell behavior underscore its significance in CRC progression. Further research is essential to elucidate precise mechanisms by which MARCHF9 enhances tumorigenesis and to explore its therapeutic potential in CRC management.

研究大肠癌中膜相关环-CH-型手指 9 的预后和致癌作用
背景和目的。结直肠癌(CRC)是一项重大的全球性健康挑战,需要对其潜在的分子机制进行全面研究,以加强诊断和治疗策略。本研究的重点是阐明膜相关环-CH型手指9(MARCHF9)--一种RING型E3泛素转移酶--在CRC中的致癌作用。我们的目的是评估 MARCHF9 的临床意义、对 CRC 进展的功能性影响及其作为预后生物标志物的潜力。研究方法我们利用癌症基因组图谱(TCGA)队列的数据评估了MARCHF9在CRC中的表达谱。体外实验包括在 COAD 细胞系(SW480 和 LoVo)中进行 siRNA 介导的 MARCHF9 基因敲除。进行了细胞增殖和侵袭试验,以研究 MARCHF9 的功能相关性。进行生存分析以评估其预后作用。结果。我们的分析表明,与正常结直肠组织相比,MARCHF9在CRC组织中的表达明显升高(P < 0.05)。MARCHF9的高表达与CRC患者的临床分期、远处转移和残留肿瘤的存在相关。生存期分析表明,MARCHF9的高表达预示着不利的总生存期和无病生存期(P < 0.05)。体外实验进一步证实了MARCHF9的致癌潜力,MARCHF9基因敲除可抑制COAD细胞的增殖和侵袭。结论这项研究揭示了MARCHF9在CRC中的致癌作用,强调了它作为潜在生物标记物和治疗靶点的临床意义。MARCHF9 与不良临床病理特征的关联及其对癌细胞行为的功能性影响强调了它在 CRC 进展中的重要性。进一步的研究对于阐明 MARCHF9 促进肿瘤发生的确切机制以及探索其在 CRC 治疗中的治疗潜力至关重要。
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来源期刊
Genetics research
Genetics research 生物-遗传学
自引率
6.70%
发文量
74
审稿时长
>12 weeks
期刊介绍: Genetics Research is a key forum for original research on all aspects of human and animal genetics, reporting key findings on genomes, genes, mutations and molecular interactions, extending out to developmental, evolutionary, and population genetics as well as ethical, legal and social aspects. Our aim is to lead to a better understanding of genetic processes in health and disease. The journal focuses on the use of new technologies, such as next generation sequencing together with bioinformatics analysis, to produce increasingly detailed views of how genes function in tissues and how these genes perform, individually or collectively, in normal development and disease aetiology. The journal publishes original work, review articles, short papers, computational studies, and novel methods and techniques in research covering humans and well-established genetic organisms. Key subject areas include medical genetics, genomics, human evolutionary and population genetics, bioinformatics, genetics of complex traits, molecular and developmental genetics, Evo-Devo, quantitative and statistical genetics, behavioural genetics and environmental genetics. The breadth and quality of research make the journal an invaluable resource for medical geneticists, molecular biologists, bioinformaticians and researchers involved in genetic basis of diseases, evolutionary and developmental studies.
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