DoSPX1 and DoMYB37 regulate the expression of DoCSLA6 in Dendrobium officinale during phosphorus starvation.

IF 5.4 3区材料科学 Q2 CHEMISTRY, PHYSICAL

ACS Applied Energy Materials Pub Date : 2024-08-26 DOI:10.1186/s12870-024-05512-8

ZhiYuan Feng, YaWen Li, SiXue Zhang, Jingjing Song, HaoXin Xiang, JunRu Huang, HongHong Fan, Lin Liu

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引用次数: 0

Abstract

Background: Dendrobium officinale Kimura et Migo (D. officinale) is parasitic on rocks or plants with very few mineral elements that can be absorbed directly, so its growth and development are affected by nutritional deficiencies. Previous studies found that phosphorus deficiency promotes polysaccharides accumulation in D. officinale, the expression of DoCSLA6 (glucomannan synthase gene) was positively correlated with polysaccharide synthesis. However, the molecular mechanism by which the low phosphorus environment affects polysaccharide accumulation remains unclear.

Results: We found that DoSPX1 can reduce phosphate accumulation in plants and promote the expression of PSIs genes, thereby enhancing plant tolerance to low phosphorus environments.Y1H and EMSA experimental show that DoMYB37 can bind the promoter of DoCSLA6. DoSPX1 interact with DoMYB37 transiently overexpressed DoSPX1 and DoMYB37 in D. officinale protocorm-like bodies, decreased the Pi content, while increased the expression of DoCSLA6.

Conclusions: The signaling pathway of DoSPX1-DoMYB37-DoCSLA6 was revealed. This provides a theoretical basis for the accumulation of polysaccharide content in D. officinale under phosphorus starvation.

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DoSPX1和DoMYB37在磷饥饿期间调控铁皮石斛中DoCSLA6的表达。

背景：Dendrobium officinale Kimura et Migo（D. officinale）寄生在岩石或植物上，可直接吸收的矿物质元素极少，因此其生长发育会受到营养缺乏的影响。之前的研究发现，缺磷会促进 D. officinale 的多糖积累，DoCSLA6（葡甘露聚糖合成酶基因）的表达与多糖合成呈正相关。然而，低磷环境影响多糖积累的分子机制仍不清楚：Y1H和EMSA实验表明，DoMYB37能与DoCSLA6的启动子结合。DoSPX1与DoMYB37相互作用在D. officinale原球茎类体中瞬时过表达DoSPX1和DoMYB37，降低了Pi含量，同时增加了DoCSLA6的表达：结论：揭示了DoSPX1-DoMYB37-DoCSLA6的信号通路。结论：揭示了DoSPX1-DoMYB37-DoCSLA6的信号通路，这为D. officinale在磷饥饿条件下多糖含量的积累提供了理论依据。

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来源期刊

ACS Applied Energy Materials Materials Science-Materials Chemistry

CiteScore

10.30

自引率

6.20%

发文量

1368

期刊介绍： ACS Applied Energy Materials is an interdisciplinary journal publishing original research covering all aspects of materials, engineering, chemistry, physics and biology relevant to energy conversion and storage. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrate knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important energy applications.