Physiological and transcriptomic profiles reveal key regulatory pathways involved in cold resistance in sunflower seedlings

IF 4.3 3区材料科学 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC

ACS Applied Electronic Materials Pub Date : 2024-08-22 DOI:10.1016/j.ygeno.2024.110926

Huifang Song , Mingyang Wang , Jie Shen , Xi Wang , Cheng Qin , Peipei Wei , Yaojun Niu , Jiahong Ren , Xiaoxue Pan , Ake Liu

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引用次数: 0

Abstract

During sunflower growth, cold waves often occur and impede plant growth. Therefore, it is crucial to study the underlying mechanism of cold resistance in sunflowers. In this study, physiological analysis revealed that as cold stress increased, the levels of ROS, malondialdehyde, ascorbic acid, and dehydroascorbic acid and the activities of antioxidant enzymes increased. Transcriptomics further identified 10,903 DEGs between any two treatments. Clustering analysis demonstrated that the expression of MYB44a, MYB44b, MYB12, bZIP2 and bZIP4 continuously upregulated under cold stress. Cold stress can induce ROS accumulation, which interacts with hormone signals to activate cold-responsive transcription factors regulating target genes involved in antioxidant defense, secondary metabolite biosynthesis, starch and sucrose metabolism enhancement for improved cold resistance in sunflowers. Additionally, the response of sunflowers to cold stress may be independent of the CBF pathway. These findings enhance our understanding of cold stress resistance in sunflowers and provide a foundation for genetic breeding.

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生理和转录组图谱揭示了参与向日葵幼苗抗寒性的关键调控途径。

在向日葵生长过程中，经常会出现寒潮，对植物生长造成不利影响。因此，研究向日葵抗寒性的内在机制至关重要。在这项研究中，生理分析表明，随着冷胁迫的增加，ROS、丙二醛、抗坏血酸和脱氢抗坏血酸的水平以及抗氧化酶的活性都会增加。转录组学进一步确定了任何两种处理之间的 10903 个 DEGs。聚类分析表明，在冷胁迫下，MYB44a、MYB44b、MYB12、bZIP2和bZIP4的表达持续上调。冷胁迫可诱导 ROS 积累，ROS 与激素信号相互作用，激活冷响应转录因子，调控涉及抗氧化防御、次生代谢物生物合成、淀粉和蔗糖代谢增强的靶基因，从而提高向日葵的抗寒性。此外，向日葵对冷胁迫的反应可能与 CBF 途径无关。这些发现加深了我们对向日葵抗寒性的了解，并为遗传育种奠定了基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Applied Electronic Materials Multiple-

CiteScore

7.20

自引率

4.30%

发文量

567