THP as a sensor for the electrochemical detection of H2O2

IF 4.5 2区 医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
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Abstract

Hydrogen peroxide (H2O2) detection is paramount in biological and clinical domains due to its pivotal role in various physiological and pathological processes. This molecule is a crucial metabolite and effector in cellular redox mechanisms, influencing diverse cellular signaling pathways and bolstering the body’s defense mechanisms against infection and oxidative stress. Organic molecule-based electrodes present unique advantages such as operational versatility and scalability, rendering them attractive candidates for sensor development across diverse fields encompassing food safety, healthcare, and environmental monitoring. This study explores the electrochemical properties of a tris(3-hydroxypyridin-4-one) THP, which has been unexplored in electrochemical sensing. Leveraging THP’s chelating properties, we aimed to develop an electrochemical probe for hydrogen peroxide detection. Our investigations reveal promising results, with the developed sensor exhibiting a low limit of detection (LOD) of 144 nM, underscoring its potential utility in sensitive and selective H2O2 detection applications. In addition, the new sensor was also tested on fetal bovine serum (FBS) to emphasize future applications on biological matrices. This research signifies a significant stride in advancing electrochemical sensor technologies for hydrogen peroxide detection with several novelties related to the usage of THP, such as high sensitivity and selectivity, performance in biological matrices, repeatability, stability, and reproducibility, economical and practical advantages. This research opens new avenues for enhanced biomedical diagnostics and therapeutic interventions.

Abstract Image

THP 作为电化学检测 H2O2 的传感器
由于过氧化氢(H2O2)在各种生理和病理过程中起着关键作用,因此过氧化氢(H2O2)检测在生物和临床领域至关重要。这种分子是细胞氧化还原机制中的重要代谢物和效应物,可影响多种细胞信号通路,并增强机体对感染和氧化应激的防御机制。基于有机分子的电极具有操作多样性和可扩展性等独特优势,是食品安全、医疗保健和环境监测等不同领域传感器开发的理想候选材料。本研究探讨了三(3-羟基吡啶-4-酮)THP 的电化学特性,该特性在电化学传感领域一直未被开发。利用 THP 的螯合特性,我们旨在开发一种用于过氧化氢检测的电化学探针。我们的研究结果很有希望,所开发的传感器的检测限(LOD)低至 144 nM,突显了其在灵敏度和选择性 H2O2 检测应用中的潜在用途。此外,新传感器还在胎牛血清(FBS)上进行了测试,以强调未来在生物基质上的应用。这项研究标志着电化学传感器技术在过氧化氢检测领域取得了重大进展,其使用的 THP 具有多种新特性,如高灵敏度和高选择性、在生物基质中的性能、可重复性、稳定性和再现性、经济性和实用性。这项研究为加强生物医学诊断和治疗干预开辟了新途径。
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来源期刊
Bioorganic Chemistry
Bioorganic Chemistry 生物-生化与分子生物学
CiteScore
9.70
自引率
3.90%
发文量
679
审稿时长
31 days
期刊介绍: Bioorganic Chemistry publishes research that addresses biological questions at the molecular level, using organic chemistry and principles of physical organic chemistry. The scope of the journal covers a range of topics at the organic chemistry-biology interface, including: enzyme catalysis, biotransformation and enzyme inhibition; nucleic acids chemistry; medicinal chemistry; natural product chemistry, natural product synthesis and natural product biosynthesis; antimicrobial agents; lipid and peptide chemistry; biophysical chemistry; biological probes; bio-orthogonal chemistry and biomimetic chemistry. For manuscripts dealing with synthetic bioactive compounds, the Journal requires that the molecular target of the compounds described must be known, and must be demonstrated experimentally in the manuscript. For studies involving natural products, if the molecular target is unknown, some data beyond simple cell-based toxicity studies to provide insight into the mechanism of action is required. Studies supported by molecular docking are welcome, but must be supported by experimental data. The Journal does not consider manuscripts that are purely theoretical or computational in nature. The Journal publishes regular articles, short communications and reviews. Reviews are normally invited by Editors or Editorial Board members. Authors of unsolicited reviews should first contact an Editor or Editorial Board member to determine whether the proposed article is within the scope of the Journal.
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