Complementary approaches to dissect late leaf rust resistance in an interspecific raspberry population.

IF 2.1 3区 生物学 Q3 GENETICS & HEREDITY
Melina Prado, Allison Vieira da Silva, Gabriela Romêro Campos, Karina Lima Reis Borges, Rafael Massahiro Yassue, Gustavo Husein, Felix Frederik Akens, Marcel Bellato Sposito, Lilian Amorim, Pariya Behrouzi, Daniela Bustos-Korts, Roberto Fritsche-Neto
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Abstract

Over the last 10 years, global raspberry production has increased by 47.89%, based mainly on the red raspberry species (Rubus idaeus). However, the black raspberry (Rubus occidentalis), although less consumed, is resistant to one of the most important diseases for the crop, the late leaf rust caused by Acculeastrum americanum fungus. In this context, genetic resistance is the most sustainable way to control the disease, mainly because there are no registered fungicides for late leaf rust in Brazil. Therefore, the aim was to understand the genetic architecture that controls resistance to late leaf rust in raspberries. For that, we used an interspecific multiparental population using the species mentioned above as parents, 2 different statistical approaches to associate the phenotypes with markers [GWAS (genome-wide association studies) and copula graphical models], and 2 phenotyping methodologies from the first to the 17th day after inoculation (high-throughput phenotyping with a multispectral camera and traditional phenotyping by disease severity scores). Our findings indicate that a locus of higher effect, at position 13.3 Mb on chromosome 5, possibly controls late leaf rust resistance, as both GWAS and the network suggested the same marker. Of the 12 genes flanking its region, 4 were possible receptors, 3 were likely defense executors, 1 gene was likely part of signaling cascades, and 4 were classified as nondefense related. Although the network and GWAS indicated the same higher effect genomic region, the network identified other different candidate regions, potentially complementing the genetic control comprehension.

用互补方法剖析树莓种间群体的晚期叶锈病抗性。
过去十年间,全球树莓产量增长了 47.89%,主要以红树莓(Rubus idaeus)为主。然而,黑树莓(Rubus occidentalis)虽然食用量较少,但对该作物最重要的病害之一,即由 Acculeastrum americanum 真菌引起的后期叶锈病具有抗性。在这种情况下,遗传抗性是控制这种病害的最可持续的方法,这主要是因为巴西没有针对晚期叶锈病的注册杀菌剂。因此,我们的目标是了解控制覆盆子对晚叶锈病抗性的遗传结构。为此,我们使用了一个以上述品种为亲本的种间多亲本群体、两种将表型与标记相关联的不同统计方法(GWAS 和 copula 图形模型)以及两种从接种后第 1 天到第 17 天的表型分析方法(使用多光谱相机进行高通量表型分析和通过疾病严重程度评分进行传统表型分析)。我们的研究结果表明,位于五号染色体 13.3 Mb 位置的一个高效应位点可能控制着晚期叶锈病的抗性,因为 GWAS 和网络都提示了相同的标记。在该区域侧翼的 12 个基因中,4 个基因可能是受体,3 个基因可能是防御执行者,1 个基因可能是信号级联的一部分,4 个基因被归类为与防御无关。虽然网络和全球基因组研究显示了相同的高效应基因组区域,但网络发现了其他不同的候选区域,有可能补充了基因控制的理解。
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来源期刊
G3: Genes|Genomes|Genetics
G3: Genes|Genomes|Genetics GENETICS & HEREDITY-
CiteScore
5.10
自引率
3.80%
发文量
305
审稿时长
3-8 weeks
期刊介绍: G3: Genes, Genomes, Genetics provides a forum for the publication of high‐quality foundational research, particularly research that generates useful genetic and genomic information such as genome maps, single gene studies, genome‐wide association and QTL studies, as well as genome reports, mutant screens, and advances in methods and technology. The Editorial Board of G3 believes that rapid dissemination of these data is the necessary foundation for analysis that leads to mechanistic insights. G3, published by the Genetics Society of America, meets the critical and growing need of the genetics community for rapid review and publication of important results in all areas of genetics. G3 offers the opportunity to publish the puzzling finding or to present unpublished results that may not have been submitted for review and publication due to a perceived lack of a potential high-impact finding. G3 has earned the DOAJ Seal, which is a mark of certification for open access journals, awarded by DOAJ to journals that achieve a high level of openness, adhere to Best Practice and high publishing standards.
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