Brightfield Multiplex Immunohistochemistry Assay for PD-L1 Evaluation in Challenging Melanoma Samples.

IF 1.3 4区 医学 Q3 ANATOMY & MORPHOLOGY
Filippo Ugolini, Luca Tinunin, Filippo Nozzoli, Sara Simi, Dario Di Gangi, Gianna Baroni, Pietro Antonini, Anna Szumera-Ciećkiewicz, Daniela Massi
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引用次数: 0

Abstract

Targeting the PD1/PD-L1 immune checkpoint pathway has rapidly become a therapeutic strategy for melanoma patients. Indeed, the quantification of PD-L1 expression by immunohistochemistry (IHC) in melanoma samples is already required, in some contexts, to allow access to anti-PD-1/PD-L1 immunotherapy. Despite a rising demand for PD-L1 testing, paralleling increasing cumulative experience in its assessment and quantification, it is fair to recognize that PD-L1 evaluation in melanoma samples still presents some critical issues. The aim of this technical report is to develop and validate a multiplex double staining protocol for PD-L1/SOX10 in Ventana Benchmark Ultra for routine practice. Our results show that double labeling provides the necessary tools to identify PD-L1 + melanoma cells clearly. The simultaneous visualization of 2 different proteins targets allows the topographical relationship between the 2 labeling to be evaluated within the context of the tissue morphology. Future studies are needed to test this technique's real-world applicability and effectiveness in implementing interpathologist agreement.

用于评估难题黑色素瘤样本中 PD-L1 的光场多重免疫组化测定。
靶向 PD1/PD-L1 免疫检查点通路已迅速成为黑色素瘤患者的一种治疗策略。事实上,在某些情况下,已经需要通过免疫组化(IHC)对黑色素瘤样本中的 PD-L1 表达进行定量,以便获得抗 PD-1/PD-L1 免疫疗法。尽管对 PD-L1 检测的需求与日俱增,其评估和量化方面的经验也在不断积累,但我们应该认识到,黑色素瘤样本中的 PD-L1 评估仍存在一些关键问题。本技术报告的目的是开发并验证用于常规操作的 Ventana Benchmark Ultra PD-L1/SOX10 多重双重染色方案。我们的结果表明,双重标记为清晰识别 PD-L1+ 黑色素瘤细胞提供了必要的工具。同时观察 2 个不同的蛋白靶点,可以在组织形态的背景下评估 2 个标记之间的地形关系。未来的研究还需要检验这项技术在现实世界中的适用性以及在病理学家之间达成一致的有效性。
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来源期刊
Applied Immunohistochemistry & Molecular Morphology
Applied Immunohistochemistry & Molecular Morphology ANATOMY & MORPHOLOGY-MEDICAL LABORATORY TECHNOLOGY
CiteScore
3.20
自引率
0.00%
发文量
153
期刊介绍: ​Applied Immunohistochemistry & Molecular Morphology covers newly developed identification and detection technologies, and their applications in research and diagnosis for the applied immunohistochemist & molecular Morphologist. Official Journal of the International Society for Immunohistochemisty and Molecular Morphology​.
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