Novel isolates of hydrogen-oxidizing chemolithoautotrophic Sulfurospirillum provide insight to the functions and adaptation mechanisms of Campylobacteria in shallow-water hydrothermal vents.

IF 5 2区 生物学 Q1 MICROBIOLOGY
mSystems Pub Date : 2024-09-17 Epub Date: 2024-08-21 DOI:10.1128/msystems.00148-24
Li Wang, Xinyi Cheng, Yizhe Guo, Junwei Cao, Mingye Sun, Jiang-Shiou Hwang, Rulong Liu, Jiasong Fang
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引用次数: 0

Abstract

Enhancing the availability of representative isolates from hydrothermal vents (HTVs) is imperative for comprehending the microbial processes that propel the vent ecosystem. In recent years, Campylobacteria have emerged as the predominant and ubiquitous taxon across both shallow and deep-sea vent systems. Nevertheless, only a few isolates have been cultured, primarily originating from deep-sea HTVs. Presently, no cultivable isolates of Campylobacteria are accessible in shallow water vent systems (<200 m), which exhibit markedly distinct environmental conditions from their deep-sea counterparts. In this study, we enriched a novel isolate (genus Sulfurospirillum, Campylobacteria) from shallow-water HTVs of Kueishan Island. Genomic and physiological analysis revealed that this novel Campylobacteria species grows on a variety of substrate and carbon/energy sources. The pan-genome and phenotypic comparisons with 12 previously isolated Sulfurospirillum species from different environments supported the identification of functional features in Sulfurospirillum genomes crucial for adaptation to vent environments, such as sulfur oxidation, carbon fixation, biofilm formation, and benzoate/toluene degradation, as well as diverse genes related with signal transportation. To conclude, the metabolic characteristics of this novel Campylobacteria augment our understanding of Campylobacteria spanning from deep-sea to shallow-water vent systems.IMPORTANCECampylobacteria emerge as the dominant and ubiquitous taxa within vent systems, playing important roles in the vent ecosystems. However, isolated representatives of Campylobacteria have been mainly from the deep-sea hydrothermal fields, leaving a significant knowledge gap regarding the functions, activities, and adaptation strategies of the vent microorganisms in shallow-water hydrothermal vents (HTVs). This study bridges this gap by providing insights into the phenomics and genomic diversity of genus Sulfurospirillum (order Campylobacterales, class Campylobacteria) based on data derived from a novel isolate obtained from shallow-water HTVs. Our mesophilic isolate of Sulfurospirillum not only augments the genus diversity of Campylobacteria pure cultures derived from vent systems but also serves as the inaugural reference isolate for Campylobacteria in shallow-water environments.

新分离的氢氧化化学溶解自养型硫螺旋体为了解浅水热液喷口弯曲杆菌的功能和适应机制提供了启示。
要了解推动喷口生态系统的微生物过程,就必须提高热液喷口(HTVs)代表性分离物的可用性。近年来,弯曲杆菌已成为浅海和深海喷口系统中普遍存在的主要分类群。然而,只有少数分离菌被培养出来,主要来自深海 HTV。目前,在浅水喷口系统中还没有从龟山岛浅水 HTV 中获得可培养的弯曲杆菌分离物(Sulfurospirillum,弯曲杆菌)。基因组和生理分析表明,这种新型弯曲杆菌可在多种基质和碳/能量源上生长。通过与之前从不同环境中分离出的 12 个螺螺旋体物种进行泛基因组和表型比较,发现了螺螺旋体基因组中对适应通风口环境至关重要的功能特征,如硫氧化、碳固定、生物膜形成和苯甲酸盐/甲苯降解,以及与信号传输相关的多种基因。总之,这种新型弯曲杆菌的代谢特征增强了我们对从深海到浅水喷口系统弯曲杆菌的了解。重要意义弯曲杆菌是喷口系统中无处不在的优势类群,在喷口生态系统中发挥着重要作用。然而,弯曲状杆菌的分离代表主要来自深海热液场,对于浅水热液喷口(HTVs)中喷口微生物的功能、活动和适应策略的了解还存在很大差距。本研究基于从浅水热液喷口获得的一个新分离株的数据,对Sulfurospirillum属(弯曲杆菌纲,弯曲杆菌属)的表型组学和基因组多样性进行了深入研究,从而弥补了这一空白。我们的 Sulfurospirillum 中嗜酸性分离株不仅增加了来自喷口系统的弯曲状杆菌纯培养物的属多样性,还可作为浅水环境中弯曲状杆菌的首次参考分离株。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
mSystems
mSystems Biochemistry, Genetics and Molecular Biology-Biochemistry
CiteScore
10.50
自引率
3.10%
发文量
308
审稿时长
13 weeks
期刊介绍: mSystems™ will publish preeminent work that stems from applying technologies for high-throughput analyses to achieve insights into the metabolic and regulatory systems at the scale of both the single cell and microbial communities. The scope of mSystems™ encompasses all important biological and biochemical findings drawn from analyses of large data sets, as well as new computational approaches for deriving these insights. mSystems™ will welcome submissions from researchers who focus on the microbiome, genomics, metagenomics, transcriptomics, metabolomics, proteomics, glycomics, bioinformatics, and computational microbiology. mSystems™ will provide streamlined decisions, while carrying on ASM''s tradition of rigorous peer review.
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