Periodontal treatment causes a longitudinal increase in nitrite-producing bacteria.

IF 2.8 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Molecular Oral Microbiology Pub Date : 2024-12-01 Epub Date: 2024-08-22 DOI:10.1111/omi.12479

Annabel Simpson, William Johnston, Miguel Carda-Diéguez, Alex Mira, Chris Easton, Fiona L Henriquez, Shauna Culshaw, Bob T Rosier, Mia Burleigh

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引用次数: 0

Abstract

Background: The oral microbiome-dependent nitrate (NO₃ ^-)-nitrite (NO₂ ^-)-nitric oxide (NO) pathway may help regulate blood pressure. NO₂ ^--producing bacteria in subgingival plaque are reduced in relative abundance in patients with untreated periodontitis compared with periodontally healthy patients. In periodontitis patients, the NO₂ ^--producing bacteria increase several months after periodontal treatment. The early effects of periodontal treatment on NO₂ ^--producing bacteria and the NO₃ ^--NO₂ ^--NO pathway remain unknown. The aim of this study was to determine how periodontal treatment affects the oral NO₂ ^--producing microbiome and salivary NO₃ ^- and NO₂ ^- levels over time.

Methods: The subgingival microbiota of 38 periodontitis patients was analysed before (baseline [BL]) and 1, 7 and 90 days after periodontal treatment. Changes in NO₂ ^--producing bacteria and periodontitis-associated bacteria were determined by 16s rRNA Illumina sequencing. Saliva samples were collected at all-time points to determine NO₃ ^- and NO₂ ^- levels using gas-phase chemiluminescence.

Results: A significant increase was observed in the relative abundance of NO₂ ^--producing species between BL and all subsequent timepoints (all p < 0.001). Periodontitis-associated species decreased at all timepoints, relative to BL (all p < 0.02). NO₂ ^--producing species negatively correlated with periodontitis-associated species at all timepoints, with this relationship strongest 90 days post-treatment (ρ = -0.792, p < 0.001). Despite these findings, no significant changes were found in salivary NO₃ ^- and NO₂ ^- over time (all p > 0.05).

Conclusions: Periodontal treatment induced an immediate increase in the relative abundance of health-associated NO₂ ^--producing bacteria. This increase persisted throughout periodontal healing. Future studies should test the effect of periodontal treatment combined with NO₃ ^- intake on periodontal and cardiovascular health.

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牙周治疗会导致产生亚硝酸盐的细菌纵向增加。

背景：口腔微生物依赖的硝酸盐（NO3-）-亚硝酸盐（NO2-）-一氧化氮（NO）途径可能有助于调节血压。与牙周健康的患者相比，未经治疗的牙周炎患者龈下菌斑中产生一氧化氮的细菌相对数量减少。牙周炎患者在牙周治疗数月后，产生 NO2 的细菌数量会增加。牙周治疗对产生 NO2 的细菌和 NO3 -NO2 -NO 途径的早期影响尚不清楚。本研究旨在确定牙周治疗如何随着时间的推移影响口腔NO2产生微生物群以及唾液中的NO3和NO2水平：方法：分析了38名牙周炎患者在牙周治疗前（基线[BL]）以及牙周治疗后1天、7天和90天的龈下微生物群。通过 16s rRNA Illumina 测序确定产生二氧化氮的细菌和牙周炎相关细菌的变化。在所有时间点采集唾液样本，使用气相化学发光法测定NO3和NO2水平：结果：在BL和随后的所有时间点之间，观察到产生NO2的物种的相对丰度明显增加（所有P 2-产生物种在所有时间点与牙周炎相关物种呈负相关，这种关系在治疗后90天最强（ρ = -0.792，P 3-和NO2-随时间变化（所有P > 0.05）：结论：牙周治疗会立即增加与健康相关的二氧化氮产生菌的相对丰度。这种增加在牙周愈合过程中持续存在。未来的研究应测试牙周治疗与三氧化二氮摄入相结合对牙周和心血管健康的影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular Oral Microbiology DENTISTRY, ORAL SURGERY & MEDICINE-MICROBIOLOGY

CiteScore

6.50

自引率

5.40%

发文量

审稿时长

>12 weeks

期刊介绍： Molecular Oral Microbiology publishes high quality research papers and reviews on fundamental or applied molecular studies of microorganisms of the oral cavity and respiratory tract, host-microbe interactions, cellular microbiology, molecular ecology, and immunological studies of oral and respiratory tract infections. Papers describing work in virology, or in immunology unrelated to microbial colonization or infection, will not be acceptable. Studies of the prevalence of organisms or of antimicrobials agents also are not within the scope of the journal. The journal does not publish Short Communications or Letters to the Editor. Molecular Oral Microbiology is published bimonthly.