A first de novo transcriptome assembly of feijoa (Acca sellowiana [Berg] Burret) reveals key genes involved in flavonoid biosynthesis.

IF 3.9 2区 生物学 Q1 GENETICS & HEREDITY
Plant Genome Pub Date : 2024-08-20 DOI:10.1002/tpg2.20501
Hector Oberti, Juan Gutierrez-Gonzalez, Clara Pritsch
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引用次数: 0

Abstract

Acca sellowiana [Berg] Burret, a cultivated fruit tree originating from South America, is gaining the attention of the nutraceutical and pharmaceutical industries due to their high content of flavonoids and other phenolic compounds in fruits, leaves, and flowers. Flavonoids are a diverse group of secondary metabolites with antioxidant, anti-inflammatory, and antimicrobial properties. They also play a crucial role in plant immune response. Despite their importance, the lack of research on A. sellowiana genomics and transcriptomics hinders a deeper understanding of the molecular mechanisms behind flavonoid biosynthesis and its regulation. Here, we de novo assembled and benchmarked 11 A. sellowiana transcriptomes from leaves and floral tissues at three developmental stages using high-throughput sequencing. We selected and annotated the best assembly according to commonly used metrics and databases. This reference transcriptome consisted of 221,649 nonredundant transcripts, of which 107,612 were functionally annotated. We then used this reference transcriptome to explore the expression profiling of key secondary metabolite genes. Transcripts from genes involved in the flavonoid and anthocyanin biosynthesis pathways were identified. We also identified 4068 putative transcription factors, with the most abundant families being bHLH, C2H2, NAC, MYB, and MYB-related. Transcript expression profiling revealed distinct patterns of gene expression during flower development. Particularly, we found 71 differentially expressed transcripts representing 14 enzymes of the flavonoid pathway, suggesting major changes in flavonoid accumulation across floral stages. Our findings will contribute to understanding the genetic basis of flavonoids and provide a foundation for further research and exploitation of the economic potential of this species.

飞燕草(Acca sellowiana [Berg] Burret)的首个全新转录组汇编揭示了参与类黄酮生物合成的关键基因。
Acca sellowiana [Berg] Burret 是一种原产于南美洲的栽培果树,由于其果实、叶片和花朵中含有大量黄酮类化合物和其他酚类化合物,因此越来越受到营养保健品和制药行业的关注。黄酮类化合物是一组种类繁多的次级代谢产物,具有抗氧化、抗炎和抗菌特性。它们在植物免疫反应中也发挥着至关重要的作用。尽管黄酮类化合物非常重要,但由于缺乏对 A. sellowiana 基因组学和转录组学的研究,我们无法深入了解黄酮类化合物生物合成及其调控背后的分子机制。在这里,我们利用高通量测序技术从新组装了11个黄花茄转录组,并对其进行了基准测试,这些转录组来自三个发育阶段的叶片和花组织。根据常用指标和数据库,我们选择并注释了最佳装配。该参考转录组包括 221,649 个非冗余转录本,其中 107,612 个已进行功能注释。然后,我们利用这一参考转录组来探索关键次生代谢物基因的表达谱。我们鉴定了参与类黄酮和花青素生物合成途径的基因转录本。我们还鉴定了 4068 个推定转录因子,其中最丰富的家族是 bHLH、C2H2、NAC、MYB 和 MYB 相关。转录表达谱分析揭示了花发育过程中不同的基因表达模式。特别是,我们发现了代表类黄酮途径14种酶的71个差异表达转录本,这表明类黄酮的积累在不同花期发生了重大变化。我们的研究结果将有助于了解类黄酮的遗传基础,并为进一步研究和开发该物种的经济潜力奠定基础。
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来源期刊
Plant Genome
Plant Genome PLANT SCIENCES-GENETICS & HEREDITY
CiteScore
6.00
自引率
4.80%
发文量
93
审稿时长
>12 weeks
期刊介绍: The Plant Genome publishes original research investigating all aspects of plant genomics. Technical breakthroughs reporting improvements in the efficiency and speed of acquiring and interpreting plant genomics data are welcome. The editorial board gives preference to novel reports that use innovative genomic applications that advance our understanding of plant biology that may have applications to crop improvement. The journal also publishes invited review articles and perspectives that offer insight and commentary on recent advances in genomics and their potential for agronomic improvement.
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