{"title":"Integrating the enzymatic syntheses of lactulose, epilactose and galacto-oligosaccharides","authors":"","doi":"10.1016/j.fbp.2024.08.002","DOIUrl":null,"url":null,"abstract":"<div><p>A mutant of cellobiose 2-epimerase from <em>Caldicellulosiruptor saccharolyticus</em> (CsCE H356N) was studied for obtaining higher yield of oligosaccharides than by lactose transgalactosylation with <em>Aspergillus oryzae</em> β-galactosidase (AO-βG). Firstly, heterologous production of CsCE H356N was optimized in Luria-Bertani broth and minimal medium. Then, CsCE H356N and AO-βG were used for the synthesis of oligosaccharides varying the sequence of reactions, temperature, and enzyme load. Reactions sequence did not affect the global yield (∼51 %) but modified the product composition. At the best conditions tested, the global yield increased twice with respect to GOS synthesis with AO-βG (25.6 %). When GOS synthesis was followed by the isomerization/epimerization of unreacted lactose, the final product had a higher content of lactulose and GOS than when the reactions sequence was inverted. In the former case lactulose-derived oligosaccharides (fGOS) and fructose were absent, while in the latter the final product mainly contained fGOS, small amounts of GOS, lactulose and fructose. Epilactose content was similar in both sequences.</p></div>","PeriodicalId":12134,"journal":{"name":"Food and Bioproducts Processing","volume":null,"pages":null},"PeriodicalIF":3.5000,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food and Bioproducts Processing","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0960308524001500","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
A mutant of cellobiose 2-epimerase from Caldicellulosiruptor saccharolyticus (CsCE H356N) was studied for obtaining higher yield of oligosaccharides than by lactose transgalactosylation with Aspergillus oryzae β-galactosidase (AO-βG). Firstly, heterologous production of CsCE H356N was optimized in Luria-Bertani broth and minimal medium. Then, CsCE H356N and AO-βG were used for the synthesis of oligosaccharides varying the sequence of reactions, temperature, and enzyme load. Reactions sequence did not affect the global yield (∼51 %) but modified the product composition. At the best conditions tested, the global yield increased twice with respect to GOS synthesis with AO-βG (25.6 %). When GOS synthesis was followed by the isomerization/epimerization of unreacted lactose, the final product had a higher content of lactulose and GOS than when the reactions sequence was inverted. In the former case lactulose-derived oligosaccharides (fGOS) and fructose were absent, while in the latter the final product mainly contained fGOS, small amounts of GOS, lactulose and fructose. Epilactose content was similar in both sequences.
期刊介绍:
Official Journal of the European Federation of Chemical Engineering:
Part C
FBP aims to be the principal international journal for publication of high quality, original papers in the branches of engineering and science dedicated to the safe processing of biological products. It is the only journal to exploit the synergy between biotechnology, bioprocessing and food engineering.
Papers showing how research results can be used in engineering design, and accounts of experimental or theoretical research work bringing new perspectives to established principles, highlighting unsolved problems or indicating directions for future research, are particularly welcome. Contributions that deal with new developments in equipment or processes and that can be given quantitative expression are encouraged. The journal is especially interested in papers that extend the boundaries of food and bioproducts processing.
The journal has a strong emphasis on the interface between engineering and food or bioproducts. Papers that are not likely to be published are those:
• Primarily concerned with food formulation
• That use experimental design techniques to obtain response surfaces but gain little insight from them
• That are empirical and ignore established mechanistic models, e.g., empirical drying curves
• That are primarily concerned about sensory evaluation and colour
• Concern the extraction, encapsulation and/or antioxidant activity of a specific biological material without providing insight that could be applied to a similar but different material,
• Containing only chemical analyses of biological materials.