Anticancer activity of naringenin on human liposarcoma: An experimental and bioinformatic study

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Applied Bio Materials Pub Date : 2024-08-21 DOI:10.1016/j.prostaglandins.2024.106884

Vahid Asghariazar , Arash Karimi , Shaghayegh Adeli , Mahtab Kadkhodayi , Erfan Zare , Mahdi vajdi , Ramin Nasimi Doost Azgoomi , Mehdi Asghari Vostakolaei

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Abstract

Naringenin (NAR) has shown potential as a cancer treatment, reducing cell proliferation and invasion in soft tissue sarcomas like liposarcoma (LPS). This study investigates NAR's role and molecular mechanism. Bioinformatic analysis was performed to assess the expression level of genes in LPS based on the GEO dataset. The heat map and PPI of genes were also analyzed. MTT, wound healing, DAPI staining, and flow cytometry evaluated the cell viability, migration, and apoptosis. Besides, real-time PCR was used to measure the NAR's impact on the expression levels of EMT, apoptosis, inflammation, and metastasis-related genes. The results showed that NAR reduces cell viability, proliferation, and migration but induces apoptosis in LPS cells. RT-PCR results revealed that NAR is capable of regulating the expression level of the apoptosis, EMT, migration, and Inflammation-related genes. This study demonstrated that NAR may play a crucial role in reducing cell viability, inducing apoptosis, and attenuating migration in Sw872 LPS cells. Consequently, NAR might be a promising and efficient factor in the treatment of LPS.

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柚皮苷对人类脂肪肉瘤的抗癌活性：一项实验和生物信息学研究

柚皮苷（NAR）具有治疗癌症的潜力，可减少脂肪肉瘤（LPS）等软组织肉瘤的细胞增殖和侵袭。本研究探讨了柚皮苷的作用和分子机制。研究人员基于 GEO 数据集进行了生物信息学分析，以评估 LPS 中基因的表达水平。同时还分析了基因的热图和 PPI。MTT、伤口愈合、DAPI 染色和流式细胞术评估了细胞活力、迁移和凋亡。此外，实时 PCR 被用来测量 NAR 对 EMT、凋亡、炎症和转移相关基因表达水平的影响。结果表明，NAR可降低LPS细胞的活力、增殖和迁移，但会诱导细胞凋亡。RT-PCR 结果显示，NAR 能够调节凋亡、EMT、迁移和炎症相关基因的表达水平。这项研究表明，NAR 在降低 Sw872 LPS 细胞的存活率、诱导细胞凋亡和减少迁移方面可能起着至关重要的作用。因此，NAR可能是一种治疗LPS的有效因子。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Applied Bio Materials Chemistry-Chemistry (all)

CiteScore

9.40

自引率

2.10%

发文量

464