Discovery, isolation, and characterization of diazeniumdiolate siderophores.

4区 生物学 Q3 Biochemistry, Genetics and Molecular Biology
Methods in enzymology Pub Date : 2024-01-01 Epub Date: 2024-07-20 DOI:10.1016/bs.mie.2024.06.006
Melanie Susman, Jin Yan, Christina Makris, Alison Butler
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引用次数: 0

Abstract

The C-diazeniumdiolate (N-nitrosohydroxylamine) group in the amino acid graminine (Gra) is a newly discovered Fe(III) ligand in microbial siderophores. Graminine was first identified in the siderophore gramibactin, and since this discovery, other Gra-containing siderophores have been identified, including megapolibactins, plantaribactin, gladiobactin, trinickiabactin (gramibactin B), and tistrellabactins. The C-diazeniumdiolate is photoreactive in UV light which provides a convenient characterization tool for this type of siderophore. This report details the process of genomics-driven identification of bacteria producing Gra-containing siderophores based on selected biosynthetic enzymes, as well as bacterial culturing, isolation and characterization of the C-diazeniumdiolate siderophores containing Gra.

发现、分离和鉴定重氮二硫酸盐苷元。
氨基酸络氨酸(Gra)中的 C-二氮二酸(N-亚硝基羟胺)基团是新发现的微生物络氨酸中的铁(III)配体。graminine最早是在嗜肽生物gramibactin中被发现的,此后又发现了其他含有Gra的嗜肽生物,包括megapolibactins、plantaribactin、gladiobactin、trinickiabactin(gramibactin B)和tistrellabactins。C-diazeniumdiolate 在紫外线下具有光活性,为这类嗜苷酸盐提供了便捷的表征工具。本报告详细介绍了根据选定的生物合成酶从基因组学角度鉴定产生含格拉的嗜苷酸盐细菌的过程,以及含格拉的 C-diazeniumdiolate 嗜苷酸盐的细菌培养、分离和表征过程。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Methods in enzymology
Methods in enzymology 生物-生化研究方法
CiteScore
2.90
自引率
0.00%
发文量
308
审稿时长
3-6 weeks
期刊介绍: The critically acclaimed laboratory standard for almost 50 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Each volume is eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with over 500 volumes the series contains much material still relevant today and is truly an essential publication for researchers in all fields of life sciences, including microbiology, biochemistry, cancer research and genetics-just to name a few. Five of the 2013 Nobel Laureates have edited or contributed to volumes of MIE.
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