Phenotypic and genomic analysis of the hypervirulent methicillin-resistant Staphylococcus aureus ST630 clone in China.

IF 5 2区生物学 Q1 MICROBIOLOGY

mSystems Pub Date : 2024-09-17 Epub Date: 2024-08-19 DOI:10.1128/msystems.00664-24

Junhong Shi, Yanghua Xiao, Li Shen, Cailing Wan, Bingjie Wang, Peiyao Zhou, Jiao Zhang, Weihua Han, Fangyou Yu

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Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) sequence type 630 (ST630) is a rarely reported lineage worldwide. This study aimed to trace the dissemination of the emerging MRSA ST630 clones in China and investigate their virulence potential. We collected 22 ST630-MRSA isolates from across China and performed whole-genome sequencing analysis and virulence characterization on these isolates. Epidemiological results showed that MRSA ST630 isolates were primarily isolated from pus/wound secretions, mainly originating from Jiangxi province, and carried diverse virulence and drug resistance genes. Staphylococcal cassette chromosome mec type V (SCCmec V) predominated (11/22, 50.0%) among the MRSA ST630 isolates. Interestingly, nearly half (45.5%) of the 22 ST630-MRSA isolates tested lacked intact SCCmec elements. Phylogenetic analysis demonstrated that ST630-MRSA could be divided into two distinct clades, with widespread dissemination mainly in Chinese regions. Five representative isolates were selected for phenotypic assays, including hemolysin activity, real-time fluorescence quantitative PCR, western blot analysis, hydrogen peroxide killing assay, blood killing assay, cell adhesion and invasion assay, and mouse skin abscess model. The results showed that, compared to the USA300-LAC strain, ST630 isolates exhibited particularly strong invasiveness and virulence in the aforementioned phenotypic assays. This study described the emergence of a highly virulent ST630-MRSA lineage and improved our insight into the molecular epidemiology of ST630 clones in China.IMPORTANCEMethicillin-resistant Staphylococcus aureus (MRSA) sequence type 630 (ST630) is an emerging clone with an increasing isolation rate in China. This study raises awareness of the hypervirulent MRSA ST630 clones in China and alerts people to their widespread dissemination. ST630-staphylococcal cassette chromosome mec V is a noteworthy clone in China, and we present the first comprehensive genetic and phenotypic analysis of this lineage. Our findings provide valuable insights for the prevention and control of infections caused by this emerging MRSA clone.

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中国高病毒耐甲氧西林金黄色葡萄球菌 ST630 克隆的表型和基因组分析。

耐甲氧西林金黄色葡萄球菌（MRSA）序列630型（ST630）是一种全球罕见的菌系。本研究旨在追踪新出现的 MRSA ST630 克隆在中国的传播情况，并研究它们的毒力潜力。我们从中国各地收集了22株ST630-MRSA分离株，并对这些分离株进行了全基因组测序分析和毒力鉴定。流行病学结果显示，MRSA ST630主要分离自脓/伤口分泌物，主要来源于江西省，携带多种毒力和耐药基因。在MRSA ST630分离株中，以金黄色葡萄球菌盒式染色体mec V型（SCCmec V）为主（11/22，50.0%）。有趣的是，在检测的 22 个 ST630-MRSA 分离物中，近一半（45.5%）缺乏完整的 SCCmec 元件。系统发生学分析表明，ST630-MRSA 可分为两个不同的支系，主要在中国地区广泛传播。研究人员选取了五个具有代表性的分离株进行表型分析，包括溶血素活性、实时荧光定量 PCR、Western 印迹分析、过氧化氢杀灭试验、血液杀灭试验、细胞粘附和侵袭试验以及小鼠皮肤脓肿模型。结果表明，与USA300-LAC菌株相比，ST630分离株在上述表型检测中表现出特别强的侵袭性和毒力。本研究描述了高致病性 ST630-MRSA 世系的出现，并提高了我们对中国 ST630 克隆的分子流行病学的认识。本研究提高了人们对中国高病毒MRSA ST630克隆的认识，并提醒人们注意其广泛传播。ST630 葡萄球菌盒式染色体 mec V 是中国值得注意的克隆，我们首次对这一菌株进行了全面的遗传和表型分析。我们的研究结果为预防和控制这种新出现的 MRSA 克隆引起的感染提供了有价值的见解。

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来源期刊

mSystems Biochemistry, Genetics and Molecular Biology-Biochemistry

CiteScore

10.50

自引率

3.10%

发文量

308

审稿时长

13 weeks

期刊介绍： mSystems™ will publish preeminent work that stems from applying technologies for high-throughput analyses to achieve insights into the metabolic and regulatory systems at the scale of both the single cell and microbial communities. The scope of mSystems™ encompasses all important biological and biochemical findings drawn from analyses of large data sets, as well as new computational approaches for deriving these insights. mSystems™ will welcome submissions from researchers who focus on the microbiome, genomics, metagenomics, transcriptomics, metabolomics, proteomics, glycomics, bioinformatics, and computational microbiology. mSystems™ will provide streamlined decisions, while carrying on ASM''s tradition of rigorous peer review.