Comparison of serum exosome miRNA from patients with Raynaud's phenomenon with positive and negative serum antinuclear antibodies.

IF 3.4 4区医学 Q2 RHEUMATOLOGY

Clinical and experimental rheumatology Pub Date : 2024-08-01 DOI:10.55563/clinexprheumatol/4iiab4

Sonsoles Piera-Velazquez, Paolo Fortina, Sergio A Jimenez

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引用次数: 0

Abstract

Objectives: To compare the microRNAs (miRNAs) contained within serum exosomes isolated from patients with Raynaud's phenomenon (RP) and negative antinuclear antibodies (ANA) to the miRNA contained in serum exosomes isolated from patients with RP and positive ANA.

Methods: Serum exosomes were isolated employing a polymer precipitation procedure. Next Generation Sequencing (NGS) was used to identify the miRNAs contained in the exosomes isolated from the two clinical cohorts and to analyse the differences in their contents.

Results: The NGS results identified six miRNAs that displayed significant differences in their content between serum exosomes from patients with RP with negative serum ANA compared to miRNAs contained in serum exosomes from patients with ANA-positive RP.

Conclusions: A comparative analysis of miRNAs contained within serum exosomes of patients with RP and negative ANA vs. samples from patients with RP and positive ANA identified several differentially expressed miRNAs that may represent non-invasive biomarkers to assist in the identification of patients with RP at risk of evolving into systemic sclerosis.

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雷诺现象患者血清外泌体 miRNA 与血清抗核抗体阳性和阴性的比较。

研究目的比较从雷诺现象（RP）和抗核抗体（ANA）阴性患者分离的血清外泌体与从雷诺现象和ANA阳性患者分离的血清外泌体中所含的microRNA（miRNA）：采用聚合物沉淀法分离血清外泌体。方法：采用聚合物沉淀法分离血清外泌体，利用新一代测序技术（NGS）鉴定从两个临床队列中分离的外泌体所含的 miRNA，并分析其含量的差异：结果：NGS结果发现，与ANA阳性RP患者血清外泌体所含的miRNA相比，血清ANA阴性RP患者血清外泌体所含的6种miRNA在含量上有显著差异：对ANA阴性的RP患者血清外泌体与ANA阳性的RP患者血清外泌体中所含的miRNA进行比较分析，发现了几种表达不同的miRNA，它们可能代表非侵入性生物标志物，有助于识别有可能演变为系统性硬化症的RP患者。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinical and experimental rheumatology 医学-风湿病学

CiteScore

6.10

自引率

18.90%

发文量

377

审稿时长

3-6 weeks

期刊介绍： Clinical and Experimental Rheumatology is a bi-monthly international peer-reviewed journal which has been covering all clinical, experimental and translational aspects of musculoskeletal, arthritic and connective tissue diseases since 1983.

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