Metabolomics to investigate the effect of preconditioned mesenchymal stem cells with crocin on pulmonary epithelial cells exposed to 2-chloroethyl ethyl sulfide

IF 2.8 2区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

Journal of proteomics Pub Date : 2024-08-13 DOI:10.1016/j.jprot.2024.105280

Vahid Jamshidi , B. Fatemeh Nobakht , Hasan Bagheri , Pardis Saeedi , Mostafa Ghanei , Raheleh Halabian

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引用次数: 0

Abstract

Metabolomics significantly impacts drug discovery and precise disease management. This study meticulously assesses the metabolite profiles of cells treated with Crocin, Dexamethasone, and mesenchymal stem cells (MSCs) under oxidative stress induced by 2-chloroethyl ethyl sulfide (CEES). Gas chromatography/mass spectrometry (GC/MS) analysis unequivocally identified substantial changes in 37 metabolites across the treated groups. Notably, pronounced alterations were observed in pathways associated with aminoacyl-tRNA biosynthesis and the metabolism of aspartate, serine, proline, and glutamate. These findings demonstrate the potent capacity of the analyzed treatments to effectively reduce inflammation, mitigate reactive oxygen species production, and enhance cell survival rates.

Significance

•
Crocin, Dexamethasone, and the metabolites of the conditioned media of mesenchymal stem cells to decline the injury caused by CEES.
•
Metabolites can acquaint treatment groups in diminishing inflammation and ROS production and expanding the percentage of cell survival.
•
Aminoacyl-tRNA biosynthesis, nitrogen metabolism, glyoxylate and dicarboxylate metabolism, and propanoate metabolism were significant pathways involved among groups.

Abstract Image

查看原文本刊更多论文

利用代谢组学研究用巴豆苷预处理间充质干细胞对暴露于 2-氯乙基硫醚的肺上皮细胞的影响。

代谢组学对药物发现和精确疾病管理具有重大影响。本研究细致评估了在2-氯乙基硫醚（CEES）诱导的氧化应激作用下，克罗欣、地塞米松和间充质干细胞（MSCs）处理细胞的代谢物谱。气相色谱/质谱（GC/MS）分析明确确定了各处理组中 37 种代谢物的重大变化。值得注意的是，在与氨基酰-tRNA 生物合成以及天冬氨酸、丝氨酸、脯氨酸和谷氨酸代谢相关的途径中观察到了明显的变化。这些研究结果表明，所分析的治疗方法能有效减轻炎症、减少活性氧的产生并提高细胞存活率。意义。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of proteomics 生物-生化研究方法

CiteScore

7.10

自引率

3.00%

发文量

227

审稿时长

73 days

期刊介绍： Journal of Proteomics is aimed at protein scientists and analytical chemists in the field of proteomics, biomarker discovery, protein analytics, plant proteomics, microbial and animal proteomics, human studies, tissue imaging by mass spectrometry, non-conventional and non-model organism proteomics, and protein bioinformatics. The journal welcomes papers in new and upcoming areas such as metabolomics, genomics, systems biology, toxicogenomics, pharmacoproteomics. Journal of Proteomics unifies both fundamental scientists and clinicians, and includes translational research. Suggestions for reviews, webinars and thematic issues are welcome.