Photodynamic therapy with curcumin and near-infrared radiation as an antitumor strategy to glioblastoma cells

IF 2.6 3区医学 Q3 TOXICOLOGY

Toxicology in Vitro Pub Date : 2024-08-12 DOI:10.1016/j.tiv.2024.105917

Marcelo Augusto Germani Marinho , Magno da Silva Marques , Camila de Oliveira Vian , Daza de Moraes Vaz Batista Filgueira , Ana Paula Horn

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引用次数: 0

Abstract

Glioblastoma is a malignant neoplasm that develops in the central nervous system and is characterized by high rates of cell proliferation and invasion, presenting resistance to treatments and a poor prognosis. Photodynamic therapy (PDT) is a therapeutic modality that can be applied in oncological cases and stands out for being less invasive. Photosensitizers (PS) of natural origin gained prominence in PDT. Curcumin (CUR) is a natural compound that has been used in PDT, considered a promising PS. In this work, we evaluated the effects of PDT-mediated CUR and near-infrared radiation (NIR) in glioblastoma cells. Through trypan blue exclusion analysis, we chose the concentration of 5 μM of CUR and the dose of 2 J/cm² of NIR that showed better responses in reducing the viable cell number in the C6 cell line and did not show cytotoxic/cytostatic effects in the HaCat cell line. Our results show that there is a positive interaction between CUR and NIR as a PDT model since there was an increase in ROS levels, a decrease in cell proliferation, increase in cytotoxicity with cell death by autophagy and necrosis, in addition to the presence of oxidative damage to proteins. These results suggest that the use of CUR and NIR is a promising strategy for the antitumor application of PDT.

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利用姜黄素和近红外辐射的光动力疗法作为胶质母细胞瘤细胞的抗肿瘤策略。

胶质母细胞瘤是一种发生在中枢神经系统的恶性肿瘤，其特点是细胞增殖率高、侵袭性强、抗药性强、预后差。光动力疗法（PDT）是一种可用于肿瘤病例的治疗模式，其突出特点是创伤较小。源自天然的光敏剂（PS）在光动力疗法中的作用日益突出。姜黄素（CUR）是一种已用于光动力疗法的天然化合物，被认为是一种很有前景的光敏剂。在这项工作中，我们评估了 CUR 和近红外辐射（NIR）在胶质母细胞瘤细胞中的光诱导疗法效果。通过胰蓝排除分析，我们选择了浓度为 5 μM 的 CUR 和剂量为 2 J/cm2 的近红外辐射，它们在减少 C6 细胞系存活细胞数方面表现出较好的反应，而在 HaCat 细胞系中未表现出细胞毒性/细胞杀伤作用。我们的研究结果表明，CUR 和近红外作为一种光紫外疗法模型之间存在着积极的相互作用，因为除了存在对蛋白质的氧化损伤外，ROS 水平增加，细胞增殖减少，细胞毒性增加，细胞通过自噬和坏死死亡。这些结果表明，使用 CUR 和近红外是一种很有前景的 PDT 抗肿瘤应用策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Toxicology in Vitro 医学-毒理学

CiteScore

6.50

自引率

3.10%

发文量

181

审稿时长

65 days

期刊介绍： Toxicology in Vitro publishes original research papers and reviews on the application and use of in vitro systems for assessing or predicting the toxic effects of chemicals and elucidating their mechanisms of action. These in vitro techniques include utilizing cell or tissue cultures, isolated cells, tissue slices, subcellular fractions, transgenic cell cultures, and cells from transgenic organisms, as well as in silico modelling. The Journal will focus on investigations that involve the development and validation of new in vitro methods, e.g. for prediction of toxic effects based on traditional and in silico modelling; on the use of methods in high-throughput toxicology and pharmacology; elucidation of mechanisms of toxic action; the application of genomics, transcriptomics and proteomics in toxicology, as well as on comparative studies that characterise the relationship between in vitro and in vivo findings. The Journal strongly encourages the submission of manuscripts that focus on the development of in vitro methods, their practical applications and regulatory use (e.g. in the areas of food components cosmetics, pharmaceuticals, pesticides, and industrial chemicals). Toxicology in Vitro discourages papers that record reporting on toxicological effects from materials, such as plant extracts or herbal medicines, that have not been chemically characterized.