Expression profiles of serum transfer RNA-derived fragments and their potential roles in non-small cell lung cancer.

IF 1.5 4区医学 Q4 ONCOLOGY

Translational cancer research Pub Date : 2024-07-31 Epub Date: 2024-07-11 DOI:10.21037/tcr-23-2364

Yulan Song, Jipeng Li

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引用次数: 0

Abstract

Background: Non-small cell lung cancer (NSCLC) is one of the malignant tumors with the highest morbidity and mortality in the world. Early diagnosis can significantly improve the prognosis of patients. Transfer RNA (tRNA)-derived fragments (tRFs) have been found to have a crucial function in the pathophysiology of cancers. However, the role of tRFs/tRNA halves (tiRNAs) in NSCLC is yet unknown. The present study aimed to investigate unique expression profiles of tRFs/tiRNAs in NSCLC and search novel biomarkers for the diagnosis.

Methods: RNA-sequencing was utilized for determining differently expressed tRFs/tiRNAs in serum in NSCLC and healthy controls. Stem-loop quantitative polymerase chain reaction (PCR) was used to confirm the selected tRFs/tiRNAs expressions. Their possible roles in NSCLC were predicted using bioinformatic research.

Results: Eleven up-regulated tRFs/tiRNAs and 18 down-regulated tRFs/tiRNAs were determined. Levels of tRF-31-87R8WP9N1EWJ0 and tRF-31-79MP9P9NH57SD were significantly higher in NSCLC serum samples than those of healthy controls; the receiver operating characteristic (ROC) curve suggested that they could serve as new diagnostic biomarkers. Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis hinted that tRF-31-87R8WP9N1EWJ0 and tRF-31-79MP9P9NH57SD might influence the development and manifestation of NSCLC.

Conclusions: In NSCLC patients' serum, the tRFs/tiRNAs were abnormally regulated and that tRF-31-87R8WP9N1EWJ0 and tRF-31-79MP9P9NH57SD might be the potential biological markers for NSCLC.

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血清转移 RNA 衍生片段的表达谱及其在非小细胞肺癌中的潜在作用。

背景：非小细胞肺癌（NSCLC非小细胞肺癌（NSCLC）是世界上发病率和死亡率最高的恶性肿瘤之一。早期诊断可大大改善患者的预后。研究发现，转运核糖核酸（tRNA）衍生片段（tRFs）在癌症的病理生理学中具有重要功能。然而，tRFs/tRNA半片（tiRNAs）在NSCLC中的作用尚不清楚。本研究旨在研究 tRFs/tiRNAs 在 NSCLC 中的独特表达谱，并寻找用于诊断的新型生物标记物：方法：利用 RNA 测序确定 NSCLC 和健康对照组血清中不同表达的 tRFs/tiRNA。采用干环定量聚合酶链反应（PCR）确认所选 tRFs/tiRNAs 的表达。通过生物信息学研究预测了它们在 NSCLC 中可能发挥的作用：结果：确定了 11 个上调 tRFs/tiRNAs 和 18 个下调 tRFs/tiRNAs。tRF-31-87R8WP9N1EWJ0和tRF-31-79MP9P9NH57SD在NSCLC血清样本中的水平显著高于健康对照组；接收者操作特征曲线（ROC）表明它们可作为新的诊断生物标志物。基因本体（GO）富集和京都基因组百科全书（KEGG）通路分析表明，tRF-31-87R8WP9N1EWJ0和tRF-31-79MP9P9NH57SD可能会影响NSCLC的发展和表现：结论：在NSCLC患者血清中，tRFs/tiRNAs被异常调控，tRF-31-87R8WP9N1EWJ0和tRF-31-79MP9P9NH57SD可能是NSCLC的潜在生物学标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Translational cancer research ONCOLOGY-

CiteScore

2.10

自引率

0.00%

发文量

252

期刊介绍： Translational Cancer Research (Transl Cancer Res TCR; Print ISSN: 2218-676X; Online ISSN 2219-6803; http://tcr.amegroups.com/) is an Open Access, peer-reviewed journal, indexed in Science Citation Index Expanded (SCIE). TCR publishes laboratory studies of novel therapeutic interventions as well as clinical trials which evaluate new treatment paradigms for cancer; results of novel research investigations which bridge the laboratory and clinical settings including risk assessment, cellular and molecular characterization, prevention, detection, diagnosis and treatment of human cancers with the overall goal of improving the clinical care of cancer patients. The focus of TCR is original, peer-reviewed, science-based research that successfully advances clinical medicine toward the goal of improving patients'' quality of life. The editors and an international advisory group of scientists and clinician-scientists as well as other experts will hold TCR articles to the high-quality standards. We accept Original Articles as well as Review Articles, Editorials and Brief Articles.