Presoaking hamstring autograft with vancomycin does not jeopardize its biomechanical properties, including graft elongation, after cyclic loading.

IF 3.3 2区医学 Q1 ORTHOPEDICS

Knee Surgery, Sports Traumatology, Arthroscopy Pub Date : 2024-08-13 DOI:10.1002/ksa.12425

Hyung Jun Park, Chan Yoon, Seung-Baik Kang, Tae Woo Kim, Chong Bum Chang, Tae Soo Bae, Moon Jong Chang, Dai-Soon Kwak

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引用次数: 0

Abstract

Purpose: Presoaking the graft with vancomycin before implantation has been shown to reduce the risk of postoperative infection after anterior cruciate ligament reconstruction (ACLR). However, the effects of presoaking on the graft biomechanical properties remain unclear. This study aimed to determine whether presoaking the graft with vancomycin affects the graft biomechanical properties and length after cyclic loading.

Methods: Ten paired (20 specimens) gracilis and semitendinous tendons were harvested from fresh-frozen human cadaveric specimens. Two tendons were folded in half to make four strands, and the grafts were randomized into the vancomycin and control groups. The graft was exposed to the antibiotic solution for 15 min (5 mg/mL) and prepared by mixing 1 g of vancomycin with 200 mL of normal saline (NaCl 0.9%). The control group was soaked in normal saline for 15 min. The prepared grafts were attached to the actuator of a dynamic tensile-testing machine. All grafts were tested with 3000 cycles of cyclic loading followed by a pull-to-failure. The cyclic loading protocol consisted of position and load control blocks to simulate the graft in vivo in the postoperative phase after ACLR.

Results: Presoaking in vancomycin did not jeopardize the biomechanical properties of the graft. In addition, presoaking with vancomycin did not elongate the grafts. No significant differences were found in the mean Young's modulus and the mean total elongation of the graft of the specimen between the vancomycin group and the control group.

Conclusion: Presoaking the graft with vancomycin jeopardized neither its biomechanical properties nor elongation even after cyclic loading in this in vitro study. It is suggested that vancomycin presoaking could be considered a safe and effective preventive measure for postoperative infections after ACLR.

Level of evidence: Not applicable.

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用万古霉素预浸泡腘绳肌自体移植物不会影响其生物力学特性，包括循环加载后移植物的伸长。

目的：研究表明，在植入前用万古霉素预浸泡移植物可降低前交叉韧带重建术（ACLR）术后感染的风险。然而，预浸泡对移植物生物力学特性的影响仍不清楚。本研究旨在确定用万古霉素预浸泡移植物是否会影响循环加载后移植物的生物力学特性和长度：方法：从新鲜冷冻的人体尸体标本中获取 10 对（20 个标本）腓肠肌和半腱肌腱。将两根肌腱对折成四股，然后将移植物随机分为万古霉素组和对照组。将 1 克万古霉素与 200 毫升生理盐水（NaCl 0.9%）混合，使移植物在抗生素溶液（5 毫克/毫升）中浸泡 15 分钟。对照组在生理盐水中浸泡 15 分钟。将制备好的移植物连接到动态拉伸试验机的推杆上。对所有移植物进行 3000 次循环加载测试，然后进行拉伸至断裂测试。循环加载方案包括位置和载荷控制块，以模拟前交叉韧带重建术后移植物在体内的情况：结果：用万古霉素进行预浸泡不会损害移植物的生物力学特性。此外，用万古霉素预浸泡也不会拉长移植物。万古霉素组与对照组样本移植物的平均杨氏模量和平均总伸长率无明显差异：结论：在这项体外研究中，用万古霉素预先浸泡移植物，即使在循环加载后也不会损害其生物力学特性或伸长率。证据级别：不适用：证据等级：不适用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Knee Surgery, Sports Traumatology, Arthroscopy 医学-外科

CiteScore

8.10

自引率

18.40%

发文量

418

审稿时长

2 months

期刊介绍： Few other areas of orthopedic surgery and traumatology have undergone such a dramatic evolution in the last 10 years as knee surgery, arthroscopy and sports traumatology. Ranked among the top 33% of journals in both Orthopedics and Sports Sciences, the goal of this European journal is to publish papers about innovative knee surgery, sports trauma surgery and arthroscopy. Each issue features a series of peer-reviewed articles that deal with diagnosis and management and with basic research. Each issue also contains at least one review article about an important clinical problem. Case presentations or short notes about technical innovations are also accepted for publication. The articles cover all aspects of knee surgery and all types of sports trauma; in addition, epidemiology, diagnosis, treatment and prevention, and all types of arthroscopy (not only the knee but also the shoulder, elbow, wrist, hip, ankle, etc.) are addressed. Articles on new diagnostic techniques such as MRI and ultrasound and high-quality articles about the biomechanics of joints, muscles and tendons are included. Although this is largely a clinical journal, it is also open to basic research with clinical relevance. Because the journal is supported by a distinguished European Editorial Board, assisted by an international Advisory Board, you can be assured that the journal maintains the highest standards. Official Clinical Journal of the European Society of Sports Traumatology, Knee Surgery and Arthroscopy (ESSKA).