Resistant starch reduces glycolysis by HK2 and suppresses high-fructose corn syrup-induced colon tumorigenesis.

IF 6.9 2区医学 Q1 GASTROENTEROLOGY & HEPATOLOGY

Journal of Gastroenterology Pub Date : 2024-10-01 Epub Date: 2024-08-14 DOI:10.1007/s00535-024-02138-3

Ying Zhang, Weiyi Shen, Zhehang Chen, Jiamin He, Lijun Feng, Lan Wang, Shujie Chen

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引用次数: 0

Abstract

Background: The intake of high-fructose corn syrup (HFCS) may increase the risk of colorectal cancer (CRC). This study aimed to explore the potential effects and mechanisms of resistant starch (RS) in HFCS-induced colon tumorigenesis.

Methods: The azoxymethane/dextran sodium sulfate (AOM/DSS) and Apc^Min/+ mice models were used to investigate the roles of HFCS and RS in CRC in vivo. An immunohistochemistry (IHC) staining analysis was used to detect the expression of proliferation-related proteins in tissues. 16S rRNA sequencing for microbial community, gas chromatography for short-chain fatty acids (SCFAs), and mass spectrometry analysis for glycolysis products in the intestines were performed. Furthermore, lactic acid assay kit was used to detect the glycolysis levels in vitro.

Results: RS suppressed HFCS-induced colon tumorigenesis through reshaping the microbial community. Mechanistically, the alteration of the microbial community after RS supplement increased the levels of intestinal SCFAs, especially butyrate, leading to the suppression of glycolysis and CRC cell proliferation by downregulating HK2.

Conclusions: Our study identified RS as a candidate of protective factors in CRC and may provide a potential target for HFCS-related CRC treatment.

Abstract Image

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抗性淀粉通过 HK2 减少糖酵解，抑制高果糖玉米糖浆诱导的结肠肿瘤发生。

背景：摄入高果糖玉米糖浆（HFCS）可能会增加罹患结直肠癌（CRC）的风险。本研究旨在探讨抗性淀粉（RS）在 HFCS 诱导的结肠肿瘤发生中的潜在作用和机制：方法：采用偶氮甲烷/葡聚糖硫酸钠（AOM/DSS）和 ApcMin/+ 小鼠模型研究 HFCS 和 RS 在体内 CRC 中的作用。免疫组化（IHC）染色分析用于检测组织中增殖相关蛋白的表达。对微生物群落进行了 16S rRNA 测序，对短链脂肪酸（SCFAs）进行了气相色谱分析，对肠道中的糖酵解产物进行了质谱分析。此外，还使用乳酸测定试剂盒检测体外糖酵解水平：结果：RS 通过重塑微生物群落抑制了 HFCS 诱导的结肠肿瘤发生。从机理上讲，补充 RS 后微生物群落的改变提高了肠道 SCFAs（尤其是丁酸盐）的水平，从而通过下调 HK2 抑制了糖酵解和 CRC 细胞的增殖：我们的研究发现 RS 是保护 CRC 的候选因子，可能为 HFCS 相关的 CRC 治疗提供潜在靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Gastroenterology 医学-胃肠肝病学

CiteScore

12.20

自引率

1.60%

发文量

审稿时长

4-8 weeks

期刊介绍： The Journal of Gastroenterology, which is the official publication of the Japanese Society of Gastroenterology, publishes Original Articles (Alimentary Tract/Liver, Pancreas, and Biliary Tract), Review Articles, Letters to the Editors and other articles on all aspects of the field of gastroenterology. Significant contributions relating to basic research, theory, and practice are welcomed. These publications are designed to disseminate knowledge in this field to a worldwide audience, and accordingly, its editorial board has an international membership.