Unravelling the evolutionary dynamics of antibiotic resistance genes in the infant gut microbiota during the first four months of life.

IF 4.6 2区 医学 Q1 MICROBIOLOGY
Anna Samarra, Raúl Cabrera-Rubio, Cecilia Martínez-Costa, Maria Carmen Collado
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引用次数: 0

Abstract

Background: Alongside microbiota development, the evolution of the resistome is crucial in understanding the early-life acquisition and persistence of Antibiotic Resistance Genes (ARGs). Therefore, the aim of this study is to provide a comprehensive view of the evolution and dynamics of the neonatal resistome from 7 days to 4 months of age using a high-throughput qPCR platform.

Methods: In the initial phase, a massive screening of 384 ARGs using a high-throughput qPCR in pooled healthy mother-infant pairs feces from the MAMI cohort was carried out to identify the most abundant and prevalent ARGs in infants and in mothers. This pre-analysis allowed for later targeted profiling in a large number of infants in a longitudinal manner during the first 4 months of life. 16S rRNA V3-V4 amplicon sequencing was performed to asses microbial composition longitudinally. Potential factors influencing the microbiota and ARGs in this period were also considered, such as mode of birth and breastfeeding type.

Results: Following the massive screening, the top 45 abundant ARGs and mobile genetic elements were identified and studied in 72 infants during their first months of life (7 days, 1, 2, and 4 months). These genes were associated with resistance to aminoglycosides, beta-lactams and tetracyclines, among others, as well as integrons, and other mobile genetic elements. Changes in both ARG composition and quantity were observed during the first 4 months of life: most ARGs abundance increased over time, but mobile genetic elements decreased significantly. Further exploration of modulating factors highlighted the effect on ARG composition of specific microbial genus, and the impact of mode of birth at 7 days and 4 months. The influence of infant formula feeding was observed at 4-month-old infants, who exhibited a distinctive resistome composition.

Conclusions: This study illustrates the ARG evolution and dynamics in the infant gut by use of a targeted, high-throughput, quantitative PCR-based method. An increase in antibiotic resistance over the first months of life were observed with a fundamental role of delivery mode in shaping resistance profiles. Further, we highlighted the influence of feeding methods on the resistome development. These findings offer pivotal insights into dynamics of and factors influencing early-life resistome, with potential avenues for intervention strategies.

揭示婴儿肠道微生物群中抗生素耐药性基因在出生后四个月内的进化动态。
背景:除了微生物群的发育,耐药基因组的进化对于了解抗生素耐药基因(ARGs)的早期获得和持久性至关重要。因此,本研究旨在利用高通量 qPCR 平台全面了解新生儿从 7 天到 4 个月大期间耐药性基因组的演变和动态:在初始阶段,研究人员使用高通量 qPCR 对来自 MAMI 队列的健康母婴粪便中的 384 个 ARGs 进行了大规模筛查,以确定婴儿和母亲体内最丰富、最普遍的 ARGs。通过这种预先分析,可以在婴儿出生后 4 个月内对大量婴儿进行有针对性的纵向分析。16S rRNA V3-V4 扩增子测序用于纵向评估微生物组成。此外,还考虑了影响这一时期微生物群和ARGs的潜在因素,如出生方式和母乳喂养类型:结果:经过大规模筛查,在 72 名婴儿出生后的头几个月(7 天、1 个月、2 个月和 4 个月)中发现并研究了含量最高的 45 个 ARGs 和移动遗传因子。这些基因与对氨基糖苷类、β-内酰胺类和四环素等药物的耐药性有关,也与整合子和其他移动遗传因子有关。在出生后的头 4 个月中,ARG 的组成和数量都发生了变化:大多数 ARG 的丰度随着时间的推移而增加,但移动遗传因子则显著减少。对调节因素的进一步探索突出了特定微生物属对 ARG 组成的影响,以及出生 7 天和 4 个月时出生方式的影响。在4个月大的婴儿身上观察到了婴儿配方奶粉喂养的影响,他们表现出了独特的抗性组组成:结论:这项研究通过使用一种基于定点、高通量、定量 PCR 的方法,说明了婴儿肠道中 ARG 的进化和动态变化。在婴儿出生后的头几个月中,我们观察到抗生素耐药性的增加,分娩方式在耐药性特征的形成中起着根本性的作用。此外,我们还强调了喂养方式对耐药性组发展的影响。这些发现为了解生命早期耐药性组的动态和影响因素提供了重要的见解,并为制定干预策略提供了潜在的途径。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
8.60
自引率
0.00%
发文量
49
审稿时长
>12 weeks
期刊介绍: Annals of Clinical Microbiology and Antimicrobials considers good quality, novel and international research of more than regional relevance. Research must include epidemiological and/or clinical information about isolates, and the journal covers the clinical microbiology of bacteria, viruses and fungi, as well as antimicrobial treatment of infectious diseases. Annals of Clinical Microbiology and Antimicrobials is an open access, peer-reviewed journal focusing on information concerning clinical microbiology, infectious diseases and antimicrobials. The management of infectious disease is dependent on correct diagnosis and appropriate antimicrobial treatment, and with this in mind, the journal aims to improve the communication between laboratory and clinical science in the field of clinical microbiology and antimicrobial treatment. Furthermore, the journal has no restrictions on space or access; this ensures that the journal can reach the widest possible audience.
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