N-acetylcysteine attenuates sodium arsenite-induced oxidative stress and apoptosis in embryonic fibroblast cells.

IF 2.2 4区 医学 Q3 TOXICOLOGY
Toxicology Research Pub Date : 2024-08-13 eCollection Date: 2024-08-01 DOI:10.1093/toxres/tfae128
Tunahan Tasci, Banu Orta-Yilmaz, Yasemin Aydin, Mahmut Caliskan
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引用次数: 0

Abstract

In recent years, the increase in environmental pollutants has been one of the most important factors threatening human and environmental health. Arsenic, a naturally occurring element found in soil, water, and air, easily enters the human body and leads to many metabolic disorders. In this study, we focused on the possible protective effects of N-acetylcysteine (NAC) against sodium arsenite (As)-induced toxic effects on embryonic fibroblast cells. The effects of As and NAC treatment on cells were evaluated, including cytotoxicity, oxidative stress, and apoptosis. Embryonic fibroblast cells were exposed to As (ranging from 0.01 μM to 10 μM) and NAC (at a concentration of 2 mM) for 24 h. The assessment of cytotoxicity markers, such as cell viability and lactate dehydrogenase (LDH), showed that As significantly reduced cell viability and increased LDH levels. Furthermore, we observed that As increased the amount of reactive oxygen species (ROS) in the cell, decreased the activity of antioxidant enzymes, and triggered apoptosis in cells. Additionally, our research revealed that the administration of NAC mitigates the detrimental effects of As. The results showed that As exerted hazardous effects on embryonic fibroblast cells through the induction of oxidative stress and apoptosis. In this context, our study provides evidence that NAC may have a protective effect against the toxicity of As in embryonic fibroblast cells.

N-乙酰半胱氨酸可减轻亚砷酸钠诱导的胚胎成纤维细胞氧化应激和凋亡。
近年来,环境污染物的增加已成为威胁人类和环境健康的最重要因素之一。砷是一种存在于土壤、水和空气中的天然元素,很容易进入人体并导致多种代谢紊乱。在这项研究中,我们重点研究了 N-乙酰半胱氨酸(NAC)对亚砷酸钠(As)诱导的胚胎成纤维细胞毒性效应的可能保护作用。我们评估了亚砷酸和 NAC 处理对细胞的影响,包括细胞毒性、氧化应激和细胞凋亡。胚胎成纤维细胞暴露于 As(浓度从 0.01 μM 到 10 μM)和 NAC(浓度为 2 mM)24 小时后,细胞毒性标志物(如细胞活力和乳酸脱氢酶(LDH))的评估结果显示,As 显著降低了细胞活力,增加了 LDH 水平。此外,我们还观察到 As 增加了细胞中活性氧(ROS)的含量,降低了抗氧化酶的活性,并引发细胞凋亡。此外,我们的研究还发现,服用 NAC 可减轻砷的有害影响。研究结果表明,砷通过诱导氧化应激和细胞凋亡对胚胎成纤维细胞产生有害影响。在这种情况下,我们的研究提供了证据,证明 NAC 可能对胚胎成纤维细胞的 As 毒性具有保护作用。
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来源期刊
Toxicology Research
Toxicology Research TOXICOLOGY-
CiteScore
3.60
自引率
0.00%
发文量
82
期刊介绍: A multi-disciplinary journal covering the best research in both fundamental and applied aspects of toxicology
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