Lectin from edible seaweed Meristotheca papulosa exhibits a high digestion-resistant property

IF 1.4 4区 农林科学 Q3 FISHERIES
Kathleen Kay Buendia, Nana Kameda-Migita, Hideyuki Teruya, Kanji Hori, Makoto Hirayama
{"title":"Lectin from edible seaweed Meristotheca papulosa exhibits a high digestion-resistant property","authors":"Kathleen Kay Buendia, Nana Kameda-Migita, Hideyuki Teruya, Kanji Hori, Makoto Hirayama","doi":"10.1007/s12562-024-01804-y","DOIUrl":null,"url":null,"abstract":"<p>The physiological impacts of polysaccharides and lipids in seaweed on human health are becoming clearer, but the behavior of the protein components, especially lectins, after ingestion remains poorly understood. In this study, we examined the resistance of edible red algae-derived lectins to digestive enzymes. We found that a lectin extracted from <i>Meristotheca papulosa</i> (MPL-1), belonging to the Jacalin-related lectin family, was relatively stable when subjected to both peptic and tryptic digestion and retained its hemagglutination activity after 24 h of digestion. The activity of MPL-1 was also maintained without a large change for 24 h following exposure to enzymes such as papain, Actinase E, and proteinase K, suggesting that MPL-1 possesses a strong resistant property against proteolytic digestion. We examined the anti-proliferation activity of the MPL fraction from the algal body against HT-29, a human colon cancer-derived cell line, and found that it showed a strong inhibitory activity with a half-maximal inhibitory concentration of 4.5 μg/mL. This activity was nullified in the presence of yeast mannan, an inhibitory sugar compound of lectin, demonstrating that MPL expressed its activity through binding to the glycan moieties on HT-29. This study indicates that this proteinase-resistant lectin could play a vital role after ingestion and is expected to have an inhibitory effect against colorectal cancer.</p>","PeriodicalId":12231,"journal":{"name":"Fisheries Science","volume":"32 1","pages":""},"PeriodicalIF":1.4000,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Fisheries Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s12562-024-01804-y","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"FISHERIES","Score":null,"Total":0}
引用次数: 0

Abstract

The physiological impacts of polysaccharides and lipids in seaweed on human health are becoming clearer, but the behavior of the protein components, especially lectins, after ingestion remains poorly understood. In this study, we examined the resistance of edible red algae-derived lectins to digestive enzymes. We found that a lectin extracted from Meristotheca papulosa (MPL-1), belonging to the Jacalin-related lectin family, was relatively stable when subjected to both peptic and tryptic digestion and retained its hemagglutination activity after 24 h of digestion. The activity of MPL-1 was also maintained without a large change for 24 h following exposure to enzymes such as papain, Actinase E, and proteinase K, suggesting that MPL-1 possesses a strong resistant property against proteolytic digestion. We examined the anti-proliferation activity of the MPL fraction from the algal body against HT-29, a human colon cancer-derived cell line, and found that it showed a strong inhibitory activity with a half-maximal inhibitory concentration of 4.5 μg/mL. This activity was nullified in the presence of yeast mannan, an inhibitory sugar compound of lectin, demonstrating that MPL expressed its activity through binding to the glycan moieties on HT-29. This study indicates that this proteinase-resistant lectin could play a vital role after ingestion and is expected to have an inhibitory effect against colorectal cancer.

Abstract Image

可食用海藻中的乳头状鱼腥草凝集素具有很强的耐消化性
海藻中的多糖和脂质对人体健康的生理影响越来越清晰,但蛋白质成分,尤其是凝集素,在摄入后的表现却仍然鲜为人知。在这项研究中,我们考察了可食用红藻凝集素对消化酶的抗性。我们发现,从乳头丝藻(Meristotheca papulosa)中提取的一种属于雅加林相关凝集素家族的凝集素(MPL-1)在消化和胰蛋白酶消化过程中都相对稳定,并且在消化 24 小时后仍能保持其血凝活性。在木瓜蛋白酶、放线菌酶 E 和蛋白酶 K 等酶的作用下,MPL-1 的活性在 24 小时内也没有发生大的变化,这表明 MPL-1 具有很强的抗蛋白酶消化的特性。我们研究了藻体中的 MPL 部分对人类结肠癌细胞株 HT-29 的抗增殖活性,发现它具有很强的抑制活性,半最大抑制浓度为 4.5 μg/mL。这种活性在酵母甘露聚糖(一种凝集素的抑制性糖化合物)的存在下无效,这表明 MPL 是通过与 HT-29 上的糖分子结合来表达其活性的。这项研究表明,这种抗蛋白酶凝集素可在摄入后发挥重要作用,并有望对结直肠癌产生抑制作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Fisheries Science
Fisheries Science 农林科学-渔业
CiteScore
3.80
自引率
5.30%
发文量
0
审稿时长
12-24 weeks
期刊介绍: Fisheries Science is the official journal of the Japanese Society of Fisheries Science, which was established in 1932. Recognized as a leading journal in its field, Fisheries Science is respected internationally for the publication of basic and applied research articles in a broad range of subject areas relevant to fisheries science. All articles are peer-reviewed by at least two experts in the field of the submitted paper. Published six times per year, Fisheries Science includes about 120 articles per volume. It has a rich history of publishing quality papers in fisheries, biology, aquaculture, environment, chemistry and biochemistry, food science and technology, and Social Science.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信