Sensitivity of Primary Human Glioblastoma Cell Lines to the Mumps Virus Vaccine Strain

IF 1.5 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecular Biology Pub Date : 2024-08-07 DOI:10.1134/s0026893324700262

E. Yu. Nikolaeva, Y. R. Zhelayeva, O. Yu. Susova, A. A. Mitrofanov, V. O. Varachev, T. V. Nasedkina, V. V. Zverev, O. A. Svitich, Y. I. Ammour

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Abstract

The sensitivity of human glioblastoma cells to virus-mediated oncolysis was investigated on five patient-derived cell lines. Primary glioblastoma cells (Gbl13n, Gbl16n, Gbl17n, Gbl25n, and Gbl27n) were infected with tenfold serial dilutions of the Leningrad-3 strain of the mumps virus, and virus reproduction and cytotoxicity were monitored for 96 −120 h. Immortalized human non-tumor NKE cells were used as controls to determine the virus specificity. Four out of the five glioblastoma cell lines examined were susceptible to mumps virus infection, whereas no virus reproduction was observed in the non-tumor cell line. Moreover, the level of proapoptotic caspase-3 activity was increased in all infected cells 48 h after infection. The kinetics of viral RNA accumulation in the studied glioblastoma cell lines was comparable with the rate of cell death. The data suggest that glioblastoma cell lines were permissive for the mumps virus. Glioblastoma cell lines differed in type I IFN production in response to the mumps virus infection. In addition, it was shown that MV infection was able to induce immunogenic death of glioblastoma cells.

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原代人类胶质母细胞瘤细胞株对腮腺炎病毒疫苗株的敏感性

摘要在五种源自患者的细胞系上研究了人类胶质母细胞瘤细胞对病毒介导的溶瘤作用的敏感性。用十倍序列稀释的列宁格勒-3株腮腺炎病毒感染原代胶质母细胞瘤细胞（Gbl13n、Gbl16n、Gbl17n、Gbl25n和Gbl27n），监测病毒繁殖和细胞毒性96-120小时。所检测的五种胶质母细胞瘤细胞系中有四种对腮腺炎病毒感染敏感，而在非肿瘤细胞系中没有观察到病毒繁殖。此外，感染 48 小时后，所有受感染细胞的促凋亡 Caspase-3 活性水平都有所提高。在所研究的胶质母细胞瘤细胞系中，病毒 RNA 的积累动力学与细胞死亡速度相当。这些数据表明，胶质母细胞瘤细胞株对腮腺炎病毒具有容许性。胶质母细胞瘤细胞系对腮腺炎病毒感染的 I 型 IFN 产生反应不同。此外，研究还表明，腮腺炎病毒感染能够诱导胶质母细胞瘤细胞免疫性死亡。

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来源期刊

Molecular Biology 生物-生化与分子生物学

CiteScore

1.30

自引率

8.30%

发文量

审稿时长

3 months

期刊介绍： Molecular Biology is an international peer reviewed journal that covers a wide scope of problems in molecular, cell and computational biology including genomics, proteomics, bioinformatics, molecular virology and immunology, molecular development biology, molecular evolution and related areals. Molecular Biology publishes reviews, experimental and theoretical works. Every year, the journal publishes special issues devoted to most rapidly developing branches of physical-chemical biology and to the most outstanding scientists.