Evaluation of genetic diversity using simple sequence repeat markers and analysis of cross compatibility in hydrangeas

IF 2.4 3区 农林科学 Q1 Agricultural and Biological Sciences
SeongHwa Bak, Tae-Ho Han
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Abstract

Hydrangeas with large inflorescences are widely used as cut flowers and in floral arrangements. However, the genetic diversity of commercially grown hydrangeas has been limited owing to the breeding focus on popular species. Therefore, this study was conducted to suggest interspecific breeding strategies for expanding the genetic diversity in Hydrangea L. by evaluating genetic diversity in the seven main species collected in Korea and analyzing cross compatibility via intra- and interspecific hybridization. Interspecific diversity evaluation using simple sequence repeat markers resulted in the segregation of 35 varieties accounting for seven species into five groups as follows: (1) Hydrangea paniculate group, (2) Hydrangea arborescnes group, (3) Hydrangea anomala and Hydrangea aspera group, (4) Hydrangea quercifolia group, and (5) Hydrangea macrophylla and Hydrangea serrata group. Cross compatibility was confirmed via intra- and interspecific crossing, and hybrids were obtained in 18 crossing combinations. Intraspecific hybrids tend to be easy to obtain, but interspecific hybrids are difficult to obtain due to a variety of factors. While most of the interspecific hybrids were obtained using ovule culture, the crossing between H. macrophylla and H. serrata created hybrids from seed sowing, indicating that H. serrata is a subspecies of H. macrophylla. Bilateral and unilateral incompatibilities were observed across the obtained hybrids. Particularly, weak bilateral compatibility was observed between H. serrata and H.paniculata. Accordingly, it was proposed that hybrids between H. macrophylla and H. serrata can be successfully used as parental materials in crossing with H. paniculata to improve cold tolerance. In addition, cross compatibility was improved in interspecific crossing using H. macrophylla and H. arborescens as maternal plants. It is anticipated that these finding will help improve the genetic diversity in commercial hydrangeas.

Abstract Image

利用简单序列重复标记评估遗传多样性并分析绣球花的杂交相容性
具有大型花序的绣球花被广泛用作切花和插花。然而,由于育种重点集中在流行品种上,商业种植绣球花的遗传多样性一直很有限。因此,本研究通过评估在韩国收集的七个主要品种的遗传多样性,并通过种内和种间杂交分析杂交相容性,提出了扩大绣球花遗传多样性的种间育种策略。利用简单序列重复标记进行的种间多样性评估将 7 个物种的 35 个品种分成以下 5 组:通过种内和种间杂交确认了杂交相容性,并在 18 个杂交组合中获得了杂交种。种内杂交往往容易获得,但种间杂交由于各种因素很难获得。虽然大多数种间杂交种都是通过胚珠培养获得的,但大叶黄杨(H. macrophylla)和大叶黄杨(H. serrata)之间的杂交从种子播种开始就产生了杂交种,这表明大叶黄杨(H. serrata)是大叶黄杨(H. macrophylla)的一个亚种。在获得的杂交种中观察到了双边和单边不亲和现象。特别是在 H. serrata 和 H.paniculata 之间观察到较弱的双边相容性。因此,研究人员提出,H. macrophylla 和 H. serrata 之间的杂交种可成功用作与 H. paniculata 杂交的亲本材料,以提高耐寒性。此外,以 H. macrophylla 和 H. arborescens 为母本的种间杂交也提高了杂交相容性。预计这些发现将有助于提高商业绣球花的遗传多样性。
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来源期刊
Horticulture Environment and Biotechnology
Horticulture Environment and Biotechnology Agricultural and Biological Sciences-Horticulture
CiteScore
4.30
自引率
4.20%
发文量
0
审稿时长
6 months
期刊介绍: Horticulture, Environment, and Biotechnology (HEB) is the official journal of the Korean Society for Horticultural Science, was launched in 1965 as the "Journal of Korean Society for Horticultural Science". HEB is an international journal, published in English, bimonthly on the last day of even number months, and indexed in Biosys Preview, SCIE, and CABI. The journal is devoted for the publication of original research papers and review articles related to vegetables, fruits, ornamental and herbal plants, and covers all aspects of physiology, molecular biology, biotechnology, protected cultivation, postharvest technology, and research in plants related to environment.
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