{"title":"Identification of Pathogen Causing Bulb Rot in Fritillaria taipaiensis P. Y. Li and Establishment of Detection Methods","authors":"Shijie Wang, Keke Chen, Jiaqi Guo, Panwang Zhang, Yuchen Li, Zhenghao Xu, Langjun Cui, Yi Qiang","doi":"10.3390/plants13162236","DOIUrl":null,"url":null,"abstract":"Fritillaria taipaiensis P. Y. Li (F. taipaiensis) is a traditional Chinese herbal medicine that has been used for over two millennia to treat cough and expectoration. However, the increasing cultivation of F. taipaiensis has led to the spread of bulb rot diseases. In this study, pathogens were isolated from rotten F. taipaiensis bulbs. Through molecular identification, pathogenicity testing, morphological assessment, and microscopy, Fusarium solani was identified as the pathogen causing bulb rot in F. taipaiensis. The colonization of F. solani in the bulbs was investigated through microscopic observation. The rapid and accurate detection of this pathogen will contribute to better disease monitoring and control. Loop-mediated isothermal amplification (LAMP) and qPCR methods were established to quickly and specifically identify this pathogen. These results provide valuable insights for further research on the prediction, rapid detection, and effective prevention and control of bulb rot in F. taipaiensis.","PeriodicalId":20103,"journal":{"name":"Plants","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2024-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plants","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3390/plants13162236","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Fritillaria taipaiensis P. Y. Li (F. taipaiensis) is a traditional Chinese herbal medicine that has been used for over two millennia to treat cough and expectoration. However, the increasing cultivation of F. taipaiensis has led to the spread of bulb rot diseases. In this study, pathogens were isolated from rotten F. taipaiensis bulbs. Through molecular identification, pathogenicity testing, morphological assessment, and microscopy, Fusarium solani was identified as the pathogen causing bulb rot in F. taipaiensis. The colonization of F. solani in the bulbs was investigated through microscopic observation. The rapid and accurate detection of this pathogen will contribute to better disease monitoring and control. Loop-mediated isothermal amplification (LAMP) and qPCR methods were established to quickly and specifically identify this pathogen. These results provide valuable insights for further research on the prediction, rapid detection, and effective prevention and control of bulb rot in F. taipaiensis.
Fritillaria taipaiensis P. Y. Li(F. taipaiensis)是一种传统中药材,用于治疗咳嗽和祛痰已有两千多年的历史。然而,随着太白金星种植量的增加,鳞茎腐烂病也随之蔓延。本研究从腐烂的太白金星鳞茎中分离出病原体。通过分子鉴定、致病性测试、形态学评估和显微镜检查,确定 Fusarium solani 是导致太白金星鳞茎腐烂病的病原体。通过显微镜观察,研究了 F. solani 在鳞茎中的定殖情况。快速准确地检测这种病原体将有助于更好地监测和控制疾病。建立了环介导等温扩增(LAMP)和 qPCR 方法,以快速、特异地鉴定这种病原体。这些结果为进一步研究如何预测、快速检测和有效防控太白金星鳞茎腐烂病提供了有价值的见解。