{"title":"Immunomodulatory Potential of Human Testicular Tissue-Derived Mesenchymal Stem/Stromal Cells over the Lifespan","authors":"Maryam Hassan Nasab, Mahmood Dehghani-Ashkezari, Fateme Montazeri, Ehsan Farashahi-Yazd, Fatemeh Hajizadeh-Tafti, Jalal Golzadeh, Seyed Mehdi Hoseini, Behrouz Aflatoonian","doi":"10.1134/s1990519x24700329","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The sequential culture expansion has been shown to affect the favorable characteristics of human mesenchymal stem/stromal cells (hMSCs), including their immunomodulatory potential. Hereby, we have investigated the immunomodulatory potential of Yazd human testicular Cells (YhTCs) regarding gene expression profile assessment during their lifespan. Morphological examination, immunostaining of vimentin and fibronectin markers, and flow cytometry analysis were used to confirm cultured cells’ MSC properties. Quantitative RT-PCR was used to investigate the target genes, <i>COX-1</i>, <i>TGF-</i>β, <i>IL-6</i>, <i>VCAM-1,</i> and <i>FAS</i>, at different passage numbers (P3, P6, and P9). Our findings confirmed the MSC properties of YhTCs over the lifespan. The results of <i>COX-1</i> and <i>IL-6</i> indicated significantly higher expression levels at the late passages (<i>p</i>‑value = 0.03 and <i>p</i>-value = 0.01, respectively). Despite the similar expression pattern, <i>VCAM-1</i> expression altered significantly at different passages (<i>p</i>-value = 0.0006), but the changes in <i>TGF-</i>β expression levels were non-significant across the lifespan. The YhTCs maintained the main characteristics of MSCs over the sequential culture expansion. Although some of the target genes in the study were sensitive to the culture expansion in the unstimulated YhTCs (<i>IL-6</i>, <i>COX-1,</i> and <i>VCAM1</i>), it seems that <i>FAS</i> and <i>TGF-</i>β need external stimuli or intracellular signals to be induced in YhTCs.</p>","PeriodicalId":9705,"journal":{"name":"Cell and Tissue Biology","volume":"22 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cell and Tissue Biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1134/s1990519x24700329","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
引用次数: 0
Abstract
The sequential culture expansion has been shown to affect the favorable characteristics of human mesenchymal stem/stromal cells (hMSCs), including their immunomodulatory potential. Hereby, we have investigated the immunomodulatory potential of Yazd human testicular Cells (YhTCs) regarding gene expression profile assessment during their lifespan. Morphological examination, immunostaining of vimentin and fibronectin markers, and flow cytometry analysis were used to confirm cultured cells’ MSC properties. Quantitative RT-PCR was used to investigate the target genes, COX-1, TGF-β, IL-6, VCAM-1, and FAS, at different passage numbers (P3, P6, and P9). Our findings confirmed the MSC properties of YhTCs over the lifespan. The results of COX-1 and IL-6 indicated significantly higher expression levels at the late passages (p‑value = 0.03 and p-value = 0.01, respectively). Despite the similar expression pattern, VCAM-1 expression altered significantly at different passages (p-value = 0.0006), but the changes in TGF-β expression levels were non-significant across the lifespan. The YhTCs maintained the main characteristics of MSCs over the sequential culture expansion. Although some of the target genes in the study were sensitive to the culture expansion in the unstimulated YhTCs (IL-6, COX-1, and VCAM1), it seems that FAS and TGF-β need external stimuli or intracellular signals to be induced in YhTCs.
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The journal publishes papers on vast aspects of cell research, including morphology, biochemistry, biophysics, genetics, molecular biology, immunology. The journal accepts original experimental studies, theoretical articles suggesting novel principles and approaches, presentations of new hypotheses, reviews highlighting major developments in cell biology, discussions. The main objective of the journal is to provide a competent representation and integration of research made on cells (animal and plant cells, both in vivo and in cell culture) offering insight into the structure and functions of live cells as a whole. Characteristically, the journal publishes articles on biology of free-living and parasitic protists, which, unlike Metazoa, are eukaryotic organisms at the cellular level of organization.
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