Evaluation of NETosis (Neutrophil Extracellular Traps) biomarkers in smokers and nonsmokers periodontitis patientsÂ

Rasha Abdul Jabbar Najim, Batool Hassan Al Ghurabi
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Abstract

Background To evaluate the NETosis biomarkers citrullinated histone H3 (citH3), neutrophil elastase (ELA), calprotectin (CALPRO), and myeloperoxidase (MPO) as indicators of inflammation in the severe stages of periodontitis III and IV in both (smokers and nonsmokers) patients, and to determine the correlation between NETosis biomarkers and clinical periodontal parameters. Methods This study recruited male subjects with an age range of (20-60) years; 60 were stage III and stage IV periodontitis patients, 30 were cigarette smoker, and 30 were nonsmokers. After applying the inclusion and exclusion criteria to evaluate their eligibility for recruitment, 25 control subjects with a healthy periodontal status and good oral hygiene maintenance were included. Unstimulated saliva was obtained and evaluated using an enzyme-linked immunosorbent assay, and the following periodontal parameters were documented: [plaque index, bleeding on probing, periodontal pocket depth, and clinical attachment loss]. Results The mean levels of all salivary NETosis biomarkers citH3, ELA, CALPRO, and MPO were elevated in the periodontitis groups (smokers and nonsmokers) than in controls. Moreover, the mean NETosis biomarker‘s mean levels were significantly higher in smoker than in nonsmokers. In addition, the correlations were significant between CALPRO and CitH3 in smokers and between ELA and CitH3 in nonsmokers. Conclusions The results of this study showed that the chosen salivary biomarkers of NETosis revealed elevated clinical accuracy in differentiating the studied periodontitis groups (smokers and nonsmokers) from controls. In addition, cigarette smoking increases the risk of periodontitis, and neutrophils in smokers with periodontitis exhibited more susceptibility to form neutrophil extracellular traps when compared with nonsmokers.
评估吸烟者和非吸烟者牙周炎患者的中性粒细胞胞外捕获器(NETosis)生物标志物
背景 目的 评估作为炎症指标的NETosis生物标志物瓜氨酸组蛋白H3(citH3)、中性粒细胞弹性蛋白酶(ELA)、钙蛋白(CALPRO)和髓过氧化物酶(MPO)在严重牙周炎III期和IV期患者(吸烟者和非吸烟者)中的作用,并确定NETosis生物标志物与临床牙周参数之间的相关性。方法 本研究招募了年龄在(20-60)岁之间的男性受试者,其中 60 人为 III 期和 IV 期牙周炎患者,30 人为吸烟者,30 人为不吸烟者。在采用纳入和排除标准评估受试者的招募资格后,还纳入了 25 名牙周健康、口腔卫生保持良好的对照组受试者。采用酶联免疫吸附测定法采集和评估未经刺激的唾液,并记录以下牙周参数:[牙菌斑指数、探诊出血、牙周袋深度和临床附着丧失]。结果 牙周炎组(吸烟者和非吸烟者)所有唾液NETosis生物标志物citH3、ELA、CALPRO和MPO的平均水平均高于对照组。此外,吸烟者的NETosis生物标志物平均水平明显高于非吸烟者。此外,吸烟者的 CALPRO 与 CitH3 之间以及非吸烟者的 ELA 与 CitH3 之间存在显著相关性。结论 本研究结果表明,所选的唾液生物标志物在区分所研究的牙周炎组(吸烟者和非吸烟者)与对照组方面具有较高的临床准确性。此外,吸烟会增加罹患牙周炎的风险,与非吸烟者相比,患有牙周炎的吸烟者体内的中性粒细胞更容易形成中性粒细胞胞外陷阱。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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