Impact of BMI and Cardiorespiratory Fitness on Oxidative Stress in Plasma and Circulating Exosomes Following Acute Exercise

Biology Pub Date : 2024-08-08 DOI:10.3390/biology13080599

Aaron L. Slusher, Nishant P Visavadiya, Brandon G. Fico, Brisamar Estébanez, Edmund O. Acevedo, Chun-Jung Huang

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Abstract

The impact of cardiorespiratory fitness (VO2max) and obesity on indices of oxidative stress in plasma and circulating exosome-like extracellular vesicles (ELVs) were examined following acute exercise. Indices of oxidative stress in plasma and isolated plasma ELVs were examined in aerobically trained (NW-Tr; n = 15) and untrained (NW-UTr; n = 18) normal-weight individuals and aerobically untrained individuals with obesity (Ob-Utr; n = 10) prior to and immediately following acute maximal treadmill running. Following exercise, ELV flotillin-1 expression (p = 0.008) and plasma total antioxidant capacity (TAC; p = 0.010) increased more in NW-UTr compared to NW-Tr and Ob-UTr participants, whereas plasma protein carbonyls (PC) decreased more in Ob-UTr compared to NW-Tr and NW-UTr groups. ELV glutathione (GSH) concentrations decreased more in NW-Tr compared to NW-UTr and Ob-UTr participants (p = 0.009), whereas lipid peroxidase (LPO) concentrations increased more in Ob-UTr compared to NW-Tr and NW-UTr participants (p = 0.003). Body mass index (BMI) was associated negatively with plasma TAC and PC (p < 0.05) and positively with ELV LPO concentration responses (p = 0.009). Finally, plasma-to-total (plasma + ELV) GSH ratios decreased in Ob-UTr compared to NW-Tr and NW-UTr participants (p = 0.006), PC ratios increased in NW-Tr and NW-UTr compared to Ob-UTr subjects (p = 0.008), and reactive oxygen/nitrogen species ratios increased in NW-UTr and decreased in Ob-UTr participants (p < 0.001). BMI, independently of VO2max, differentially regulates indices of oxidative stress within plasma and circulating ELVs prior to and immediately following acute maximal treadmill exercise.

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急性运动后体重指数和心肺功能对血浆和循环外泌体中氧化应激的影响

研究人员考察了急性运动后心肺功能（VO2max）和肥胖对血浆和循环外泌体类细胞外囊泡（ELVs）中氧化应激指数的影响。有氧训练（NW-Tr；n = 15）和无氧训练（NW-UTr；n = 18）的正常体重者和无氧训练的肥胖者（Ob-Utr；n = 10）在急性最大跑步机跑步之前和之后，血浆和分离血浆ELV中的氧化应激指数进行了检测。运动后，与NW-Tr组和Ob-UTr组相比，NW-UTr组的ELV flotillin-1表达（p = 0.008）和血浆总抗氧化能力（TAC；p = 0.010）增加得更多，而与NW-Tr组和NW-UTr组相比，Ob-UTr组的血浆蛋白质羰基（PC）减少得更多。NW-Tr组与NW-UTr组和Ob-UTr组相比，ELV谷胱甘肽（GSH）浓度下降较多（p = 0.009），而Ob-UTr组与NW-Tr组和NW-UTr组相比，脂质过氧化物酶（LPO）浓度增加较多（p = 0.003）。体重指数（BMI）与血浆 TAC 和 PC 呈负相关（p < 0.05），与 ELV LPO 浓度反应呈正相关（p = 0.009）。最后，与 NW-Tr 和 NW-UTr 参与者相比，Ob-UTr 参与者的血浆与总 GSH（血浆 + ELV）比率降低（p = 0.006），与 Ob-UTr 参与者相比，NW-Tr 和 NW-UTr 参与者的 PC 比率升高（p = 0.008），NW-UTr 参与者的活性氧/氮物种比率升高，而 Ob-UTr 参与者的活性氧/氮物种比率降低（p < 0.001）。体重指数与最大氧饱和度无关，在急性最大跑步机运动前和运动后会对血浆和循环ELV中的氧化应激指数产生不同的调节作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Biology

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