Alteration of Photosynthetic and Antioxidant Gene Expression in Sugarcane Infected by Multiple Mosaic Viruses

Q4 Agricultural and Biological Sciences

International Journal of Plant Biology Pub Date : 2024-08-08 DOI:10.3390/ijpb15030055

Intan Ria Neliana, Wardatus Soleha, Suherman, Nurmalasari Darsono, R. Harmoko, W. D. Sawitri, Bambang Sugiharto

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引用次数: 0

Abstract

Sugarcane mosaic virus (SCMV), sugarcane streak mosaic virus (SCSMV), and sorghum mosaic virus (SrMV) are the causative pathogens of mosaic disease. This study aimed to identify mosaic virus infection and its impact on photosynthetic and antioxidant gene expression in eight commercial sugarcane cultivars grown on sugarcane plantations in East Java, Indonesia. The disease incidence and severity were observed in symptomatic leave samples, and then the virus was identified. A high incidence and severity of mosaic symptoms were observed in the PS881 and NX04 cultivars compared with the other cultivars. RT-PCR analysis detected SCSMV infection in all cultivars; double infections with SCSMV and SCMV in the PS881, PS882, and Cening cultivars; and triple infections with SCSMV, SCMV, and SrMV in the PS881 cultivar. Ascorbate peroxidase (Apx) expression was upregulated in all virus-infected cultivars and significantly increased in the triple-infected PS881 cultivar. However, catalase (Cat) expression was only slightly increased in the PS881 cultivar. The chlorophyll content was reduced, and the PsaA gene was downregulated in all cultivars. The expression of PsaA, RbcS, and Sps was significantly suppressed in the triple-infected PS881 cultivar. Moreover, the downregulation of both the RbcS and Pepc genes was concomitant with that of their protein levels.

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受多种马赛克病毒感染的甘蔗中光合和抗氧化基因表达的变化

甘蔗马赛克病毒（SCMV）、甘蔗条斑马赛克病毒（SCSMV）和高粱马赛克病毒（SrMV）是马赛克病的病原体。本研究旨在确定印度尼西亚东爪哇甘蔗种植园种植的八个商业甘蔗栽培品种的马赛克病毒感染情况及其对光合作用和抗氧化基因表达的影响。在有症状的叶片样本中观察了病害的发生率和严重程度，然后对病毒进行了鉴定。与其他栽培品种相比，PS881 和 NX04 的马赛克症状发生率和严重程度较高。RT-PCR 分析检测到所有栽培品种都感染了 SCSMV；PS881、PS882 和 Cening 栽培品种感染了 SCSMV 和 SCMV 双重感染；PS881 栽培品种感染了 SCSMV、SCMV 和 SrMV 三重感染。在所有受病毒感染的栽培品种中，抗坏血酸过氧化物酶（Apx）的表达均上调，而在受三重感染的 PS881 栽培品种中，抗坏血酸过氧化物酶（Apx）的表达显著增加。然而，过氧化氢酶（Cat）的表达在 PS881 栽培品种中仅略有增加。叶绿素含量降低，PsaA 基因在所有栽培品种中都出现下调。在三重感染的 PS881 栽培品种中，PsaA、RbcS 和 Sps 的表达明显受到抑制。此外，RbcS 和 Pepc 基因的下调与其蛋白质水平的下调同时发生。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Plant Biology Agricultural and Biological Sciences-Plant Science

CiteScore

2.00

自引率

0.00%

发文量

审稿时长

10 weeks

期刊介绍： The International Journal of Plant Biology is an Open Access, online-only, peer-reviewed journal that considers scientific papers in all different subdisciplines of plant biology, such as physiology, molecular biology, cell biology, development, genetics, systematics, ecology, evolution, ecophysiology, plant-microbe interactions, mycology and phytopathology.