Antifungal Effect of Poly(methyl methacrylate) with Farnesol and Undecylenic Acid against Candida albicans Biofilm Formation

Materials Pub Date : 2024-08-08 DOI:10.3390/ma17163936

Milica Išljamović, D. Bonvin, Milena Milojević, Simona Stojanović, M. Spasić, B. Stojković, P. Janošević, Suzana A Otasevic, M. M. Ebersold

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Abstract

The control of Candida albicans biofilm formation on dentures made of poly(methyl methacrylate) (PMMA) is an important challenge due to the high resistance to antifungal drugs. Interestingly, the natural compounds undecylenic acid (UDA) and farnesol (FAR) both prevent C. albicans biofilm formation and could have a synergetic effect. We therefore modified PMMA with a combination of UDA and FAR (UDA+FAR), aiming to obtain the antifungal PMMA_UDA+FAR composites. Equal concentrations of FAR and UDA were added to PMMA to reach 3%, 6%, and 9% in total of both compounds in composites. The physico-chemical properties of the composites were characterized by Fourier-transform infrared spectroscopy and water contact angle measurement. The antifungal activity of the composites was tested on both biofilm and planktonic cells with an XTT test 0 and 6 days after the composites’ preparation. The effect of the UDA+FAR combination on C. albicans filamentation was studied in agar containing 0.0125% and 0.4% UDA+FAR after 24 h and 48 h of incubation. The results showed the presence of UDA and FAR on the composite and decreases in the water contact angle and metabolic activity of both the biofilm and planktonic cells at both time points at non-toxic UDA+FAR concentrations. Thus, the modification of PMMA with a combination of UDA+FAR reduces C. albicans biofilm formation on dentures and could be a promising anti-Candida strategy.

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含有法呢醇和十一烯酸的聚甲基丙烯酸甲酯对白色念珠菌生物膜形成的抗真菌作用

聚甲基丙烯酸甲酯（PMMA）制成的假牙对抗真菌药物具有很强的耐药性，因此如何控制假牙上白色念珠菌生物膜的形成是一项重要的挑战。有趣的是，天然化合物十一碳烯酸（UDA）和法呢醇（FAR）都能阻止白僵菌生物膜的形成，并能产生协同效应。因此，我们用 UDA 和 FAR（UDA+FAR）的组合对 PMMA 进行改性，旨在获得抗真菌的 PMMA_UDA+FAR 复合材料。在 PMMA 中添加等浓度的 FAR 和 UDA，使两种化合物在复合材料中的总含量分别达到 3%、6% 和 9%。通过傅立叶变换红外光谱和水接触角测量对复合材料的物理化学性质进行了表征。在制备复合材料 0 天和 6 天后，通过 XTT 试验测试了复合材料对生物膜细胞和浮游细胞的抗真菌活性。在含有 0.0125% 和 0.4% UDA+FAR 的琼脂中培养 24 小时和 48 小时后，研究了 UDA+FAR 组合对白僵菌丝状化的影响。结果表明，在无毒的 UDA+FAR 浓度下，复合材料上存在 UDA 和 FAR，并且在两个时间点上，生物膜和浮游细胞的水接触角和代谢活性都有所下降。因此，用 UDA+FAR 组合改性 PMMA 可以减少假牙上白念珠菌生物膜的形成，是一种很有前景的抗念珠菌策略。

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Materials

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