Intestinal Dysbiosis, Tight Junction Proteins, and Inflammation in Rheumatoid Arthritis Patients: A Cross-Sectional Study

IF 4.9 2区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

International Journal of Molecular Sciences Pub Date : 2024-08-08 DOI:10.3390/ijms25168649

A. Mucientes, J. M. Lisbona-Montañez, N. Mena-Vázquez, Patricia Ruiz-Limón, S. Manrique-Arija, Aimara García-Studer, F. Ortiz-Márquez, Antonio Fernández-Nebro

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Abstract

Recent studies point to intestinal permeability as an important factor in the establishment and development of rheumatoid arthritis (RA). Tight junctions (TJs) play a major role in intestinal homeostasis. The alteration of this homeostasis is related to RA. Furthermore, RA patients present dysbiosis and a lower microbiota diversity compared to healthy individuals. A cross-sectional study including RA patients and sex- and age-matched healthy controls was performed. The quantification of TJ proteins was carried out by ELISA. Gut microbiota was evaluated by NGS platform Ion Torrent S. The inflammatory variables included were DAS28, CRP, inflammatory cytokines (IL-6, IL-1, TNF-α) and oxidised LDL. Claudin-1 levels showed significant differences between groups. Results evidenced a correlation between claudin-1 values and age (r: −0.293; p < 0.05), IL6 (r: −0.290; p < 0.05) and CRP (r: −0.327; p < 0.05), and between zonulin values and both age (r: 0.267; p < 0.05) and TNFα (r: 0.266; p < 0.05). Moreover, claudin-1 and CRP levels are related in RA patients (β: −0.619; p: 0.045), and in patients with high inflammatory activity, the abundance of the genus Veillonella is positively associated with claudin-1 levels (β: 39.000; p: 0.004).

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类风湿性关节炎患者的肠道菌群失调、紧密连接蛋白和炎症：一项横断面研究

最近的研究表明，肠道通透性是类风湿性关节炎（RA）发病和发展的一个重要因素。紧密连接（TJ）在肠道平衡中发挥着重要作用。这种平衡的改变与 RA 有关。此外，与健康人相比，RA 患者的肠道菌群失调，微生物群多样性较低。一项横断面研究包括了 RA 患者以及性别和年龄匹配的健康对照组。采用 ELISA 方法对 TJ 蛋白进行定量。炎症变量包括 DAS28、CRP、炎症细胞因子（IL-6、IL-1、TNF-α）和氧化低密度脂蛋白。Claudin-1水平在不同组间存在显著差异。结果表明，Claudin-1值与年龄（r：-0.293；p＜0.05）、IL6（r：-0.290；p＜0.05）和CRP（r：-0.327；p＜0.05）相关，zonulin值与年龄（r：0.267；p＜0.05）和TNFα（r：0.266；p＜0.05）相关。此外，在 RA 患者中，claudin-1 和 CRP 水平相关（β：-0.619；p：0.045），在炎症活动较强的患者中，Veillonella 属的丰度与 claudin-1 水平呈正相关（β：39.000；p：0.004）。

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来源期刊

International Journal of Molecular Sciences Chemistry-Organic Chemistry

CiteScore

8.10

自引率

10.70%

发文量

13472

审稿时长

17.49 days

期刊介绍： The International Journal of Molecular Sciences (ISSN 1422-0067) provides an advanced forum for chemistry, molecular physics (chemical physics and physical chemistry) and molecular biology. It publishes research articles, reviews, communications and short notes. Our aim is to encourage scientists to publish their theoretical and experimental results in as much detail as possible. Therefore, there is no restriction on the length of the papers or the number of electronics supplementary files. For articles with computational results, the full experimental details must be provided so that the results can be reproduced. Electronic files regarding the full details of the calculation and experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material (including animated pictures, videos, interactive Excel sheets, software executables and others).