Exopolysaccharide is required by Paraburkholderia phytofirmans PsJN to confer drought-stress tolerance in pea

C. Prihatna, Qing Yan
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Abstract

Paraburkholderia phytofirmans PsJN is a plant symbiotic bacterium that can colonize a broad spectrum of plant hosts and frequently shows beneficial effects on plant growth. Exopolysaccharide (EPS) is known to be important in plant-bacteria interactions. Previously, we reported that EPS is required for PsJN to survive from drought stress and colonize in pea (Pisum sativum) under drought condition. However, whether EPS is necessary for PsJN to promote plant growth remains unknown. In this work, a comparative study was conducted between the wild-type PsJN and its ∆bceQ mutant that lacks EPS to investigate the role of EPS in PsJN to confer drought-stress tolerance on pea plant. Our results showed that wild type PsJN, but not the ∆bceQ mutant, promoted pea seed germination and seedlings growth under drought stress. Pea plants inoculated with the wild type PsJN had a higher level of drought tolerance, as shown by a better vegetative growth and enhanced nodule formation, than plants inoculated with the ∆bceQ mutant. Moreover, EPS plays a role in the plant colonization under drought stress, because the ∆bceQ mutant was unable to colonize pea seeds and roots as effectively as the wild type PsJN. Further, expression of the EPS biosynthesis genes in the bceOVN operon of the wild type PsJN was induced by the presence of glucose. Overall, this study demonstrated that PsJN can promote pea plant growth under drought conditions and EPS is required for PsJN to confer beneficial effects to host plant.
Paraburkholderia phytofirmans PsJN需要外多糖来赋予豌豆抗旱能力
Paraburkholderia phytofirmans PsJN 是一种植物共生细菌,可定植于多种植物宿主,并经常对植物生长产生有益影响。众所周知,外多糖(EPS)在植物与细菌的相互作用中非常重要。此前,我们曾报道 PsJN 在干旱条件下从干旱胁迫中存活并在豌豆(Pisum sativum)中定殖需要 EPS。然而,EPS 是否是 PsJN 促进植物生长的必要条件仍是未知数。本研究对野生型 PsJN 及其缺乏 EPS 的突变体 ∆bceQ 进行了比较研究,以探讨 EPS 在 PsJN 赋予豌豆植物抗旱性中的作用。结果表明,野生型 PsJN(而非 ∆bceQ 突变体)在干旱胁迫下能促进豌豆种子萌发和幼苗生长。与接种了 ∆bceQ 突变体的植株相比,接种了野生型 PsJN 的豌豆植株具有更高的耐旱性,表现为植株生长更好,结瘤形成更强。此外,EPS 在干旱胁迫下的植物定殖过程中也发挥了作用,因为 ∆bceQ 突变体无法像野生型 PsJN 那样有效地在豌豆种子和根部定殖。此外,野生型 PsJN 的 bceOVN 操作子中的 EPS 生物合成基因的表达受到葡萄糖的诱导。总之,本研究证明了 PsJN 能在干旱条件下促进豌豆植物的生长,而 EPS 是 PsJN 赋予宿主植物有益效应所必需的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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