Analysis of the Brucella melitensis epidemic in Xinjiang: genotyping, polymorphism, antibiotic resistance and tracing.

IF 4.6 2区 医学 Q1 MICROBIOLOGY
Xiaowen Yang, Yan Liu, Na Li, Xiaowei Peng, Yinghui Zhang, Xiaoqian Zhang, Lin Liang, Zengjie Bian, Hui Jiang, Jiabo Ding
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引用次数: 0

Abstract

Brucella spp. are facultative intracellular pathogens that cause zoonosis- brucellosis worldwide. There has been a trend of the re-emergence of brucellosis worldwide in recent years. The epidemic situation of brucellosis is serious in Xinjiang. To analyze the epidemic situation of Brucella spp. in Xinjiang among humans and animals, this study identified 144 Brucella isolates from Xinjiang using classical identification and 16 S rRNA sequencing. MLVA, drug resistance testing, and wgSNP detection were also performed. At the same time, analysis was conducted based on the published data of Brucella isolates worldwide. The results showed that the dominant species was B. melitensis biovar 3, which belonged to GT42 (MLVA-8 typing) and the East Mediterranean lineage. The correlation among isolates was high both in humans or animals. The isolates in Xinjiang exhibited higher polymorphism compared to other locations in China, with polymorphism increasing each year since 2010. No amikacin/kanamycin-resistant strains were detected, but six rifampicin-intermediate isolates were identified without rpoB gene variation. The NJ tree of the wgSNP results indicated that there were three main complexes of the B. melitensis epidemic in Xinjiang. Based on the results of this study, the prevention and control of brucellosis in Xinjiang should focus on B. melitensis, particularly strains belonging to B. melitensis bv.3 GT42 (MLVA-8 typing) and East Mediterranean lineage. Additionally, the rifampicin- and trimethoprim-sulfamethoxazole- resistance of isolates in Xinjiang should be closely monitored to avoid compromising the therapeutic efficacy and causing greater losses. These results provide essential data for the prevention and control of brucellosis in Xinjiang and China. Although the isolates from Xinjiang have significant characteristics among Chinese isolates and can reflect the epidemiological situation of brucellosis in China to some extent, this study cannot represent the characteristics of isolates from other regions.

新疆布鲁氏菌疫情分析:基因分型、多态性、抗生素耐药性和溯源。
布鲁氏菌属是一种表面性细胞内病原体,可在全球范围内引起人畜共患病--布鲁氏菌病。近年来,布鲁氏菌病在世界范围内有重新抬头的趋势。新疆布鲁氏菌病流行形势严峻。为了分析布鲁氏菌在新疆人畜中的流行情况,本研究采用经典鉴定和 16 S rRNA 测序方法鉴定了新疆的 144 株布鲁氏菌分离物。还进行了 MLVA、耐药性测试和 wgSNP 检测。同时,还根据已公布的全球布鲁氏菌分离物数据进行了分析。结果表明,布鲁氏菌的优势种是梅里金布鲁氏菌生物变种 3,属于 GT42(MLVA-8 分型)和东地中海系。无论是人还是动物,分离株之间的相关性都很高。与中国其他地区相比,新疆的分离株表现出更高的多态性,自2010年以来,多态性逐年增加。没有发现耐阿米卡星/卡那霉素的菌株,但发现了 6 株无 rpoB 基因变异的利福平中型分离株。wgSNP 结果的 NJ 树表明,新疆的梅毒杆菌疫情有三个主要复合体。根据本研究结果,新疆布鲁氏菌病的防控重点应放在布鲁氏菌上,尤其是属于布鲁氏菌bv.3 GT42(MLVA-8分型)和东地中海系的菌株。此外,应密切监测新疆分离株对利福平和三甲双氨-磺胺甲噁唑的耐药性,以免影响疗效,造成更大的损失。这些结果为新疆和中国的布鲁氏菌病防控工作提供了重要数据。尽管新疆分离株在中国分离株中具有显著特征,能在一定程度上反映中国布鲁氏菌病的流行状况,但本研究不能代表其他地区分离株的特征。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
8.60
自引率
0.00%
发文量
49
审稿时长
>12 weeks
期刊介绍: Annals of Clinical Microbiology and Antimicrobials considers good quality, novel and international research of more than regional relevance. Research must include epidemiological and/or clinical information about isolates, and the journal covers the clinical microbiology of bacteria, viruses and fungi, as well as antimicrobial treatment of infectious diseases. Annals of Clinical Microbiology and Antimicrobials is an open access, peer-reviewed journal focusing on information concerning clinical microbiology, infectious diseases and antimicrobials. The management of infectious disease is dependent on correct diagnosis and appropriate antimicrobial treatment, and with this in mind, the journal aims to improve the communication between laboratory and clinical science in the field of clinical microbiology and antimicrobial treatment. Furthermore, the journal has no restrictions on space or access; this ensures that the journal can reach the widest possible audience.
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