Two cyanobacterial species exhibit stress responses when grown together in visible light or far-red light.

IF 3.7 2区 生物学 Q2 MICROBIOLOGY
mSphere Pub Date : 2024-09-25 Epub Date: 2024-08-09 DOI:10.1128/msphere.00251-24
Ting-Shuo Nien, Ting-Hsuan Chan, Ying-Yang Li, Ting-So Liu, Yo-Jin Shiau, Ming-Yang Ho
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引用次数: 0

Abstract

Although most cyanobacteria grow in visible light (VL; λ = 400-700 nm), some cyanobacteria can also use far-red light (FRL; λ = 700-800 nm) for oxygenic photosynthesis by performing far-red light photoacclimation. These two types of cyanobacteria can be found in the same environment. However, how they respond to each other remains unknown. Here, we reveal that coculture stresses FRL-using Chlorogloeopsis fritschii PCC 9212 and VL-using Synechocystis sp. PCC 6803. No significant growth difference was found in Synechocystis sp. PCC 6803 between the coculture and the monoculture. Conversely, the growth of Chlorogloeopsis fritschii PCC 9212 was suppressed in VL under coculture. According to transcriptomic analysis, Chlorogloeopsis fritschii PCC 9212 in coculture shows low transcript levels of metabolic activities and high transcript levels of ion transporters, with the differences being more noticeable in VL than in FRL. The transcript levels of stress responses in coculture were likewise higher than in monoculture in Synechocystis sp. PCC 6803 under FRL. The low transcript level of metabolic activities in coculture or the inhibition of cyanobacterial growth indicates a possible negative interaction between these two cyanobacterial strains.IMPORTANCEThe interaction between two cyanobacterial species is the primary focus of this study. One species harvests visible light, while the other can harvest far-red and visible light. Prior research on cyanobacteria interaction concentrated on its interactions with algal, coral, and fungal species. Interactions between cyanobacterial species were, nevertheless, rarely discussed. Thus, we characterized the interaction between two cyanobacterial species, one capable of photosynthesis using far-red light and the other not. Through experimental and bioinformatic approaches, we demonstrate that when one cyanobacterium thrives under optimal light conditions, it stresses the remaining cyanobacterial species. We contribute to an ecological understanding of these two kinds of cyanobacteria distribution patterns. Cyanobacteria that utilize far-red light probably disperse in environments with limited visible light to avoid competition with other cyanobacteria. From a biotechnological standpoint, this study suggests that the simultaneous cultivation of two cyanobacterial species in large-scale cultivation facilities may reduce the overall biomass yield.

当两种蓝藻在可见光或远红光下一起生长时,会出现应激反应。
虽然大多数蓝藻在可见光(VL;λ = 400-700 纳米)下生长,但有些蓝藻也能通过远红光光适应(FRL;λ = 700-800 纳米)进行含氧光合作用。这两种蓝藻可以在同一环境中发现。然而,它们如何相互反应仍是未知数。在此,我们揭示了共培养对使用 FRL 的 Chlorogloeopsis fritschii PCC 9212 和使用 VL 的 Synechocystis sp.Synechocystis sp. PCC 6803 在共培养和单培养之间没有发现明显的生长差异。相反,在共培养条件下,Chlorogloeopsis fritschii PCC 9212 在 VL 中的生长受到抑制。根据转录组分析,在共培养条件下,Chlorogloeopsis fritschii PCC 9212 的代谢活动转录水平较低,而离子转运体转录水平较高,在 VL 中的差异比在 FRL 中更明显。同样,在 FRL 条件下,共培养 Synechocystis sp.两种蓝藻之间的相互作用是本研究的主要重点。一种蓝藻收获可见光,而另一种蓝藻则能收获远红光和可见光。之前有关蓝藻相互作用的研究主要集中在蓝藻与藻类、珊瑚和真菌的相互作用上。然而,蓝藻物种之间的相互作用却很少被讨论。因此,我们研究了两种蓝藻之间的相互作用,一种能利用远红光进行光合作用,另一种则不能。通过实验和生物信息学方法,我们证明了当一种蓝藻在最佳光照条件下茁壮成长时,会对其余蓝藻物种造成压力。我们的研究有助于从生态学角度理解这两种蓝藻的分布模式。利用远红光的蓝藻可能会分散在可见光有限的环境中,以避免与其他蓝藻竞争。从生物技术的角度来看,这项研究表明,在大规模栽培设施中同时栽培两种蓝藻可能会降低总体生物量产量。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
mSphere
mSphere Immunology and Microbiology-Microbiology
CiteScore
8.50
自引率
2.10%
发文量
192
审稿时长
11 weeks
期刊介绍: mSphere™ is a multi-disciplinary open-access journal that will focus on rapid publication of fundamental contributions to our understanding of microbiology. Its scope will reflect the immense range of fields within the microbial sciences, creating new opportunities for researchers to share findings that are transforming our understanding of human health and disease, ecosystems, neuroscience, agriculture, energy production, climate change, evolution, biogeochemical cycling, and food and drug production. Submissions will be encouraged of all high-quality work that makes fundamental contributions to our understanding of microbiology. mSphere™ will provide streamlined decisions, while carrying on ASM''s tradition for rigorous peer review.
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