In vitro impacts of polystyrene microplastics and environmental pollutants on ethoxyresorufin-O-deethylase and glutathione S-transferase activity in Mossambica tilapia

IF 2.6 3区医学 Q3 TOXICOLOGY

Toxicology in Vitro Pub Date : 2024-08-05 DOI:10.1016/j.tiv.2024.105915

Yan Yin , Haiyan Wang , Guijing Ouyang , Daxiong Han

{"title":"In vitro impacts of polystyrene microplastics and environmental pollutants on ethoxyresorufin-O-deethylase and glutathione S-transferase activity in Mossambica tilapia","authors":"Yan Yin , Haiyan Wang , Guijing Ouyang , Daxiong Han","doi":"10.1016/j.tiv.2024.105915","DOIUrl":null,"url":null,"abstract":"<div>Microplastic (MP) pollution is a potential threat to marine organisms. In vitro toxicity of MPs and other pollutants, such as pharmaceutically active compounds (PhACs) and brominated flame retardants (BFRs), has been understudied. This study aimed to investigate the effects of polystyrene microplastics (PS-MPs) with different particle sizes on two biomarkers: ethoxyresorufin-O-deethylase (EROD) and glutathione S-transferase (GST) in tilapia liver homogenates. The study also examined the combined effects of PS-MPs with various environmental contaminants, including three metal ions (Cu2+, Zn2+, Pb2+), three BFRs, and six PhACs. PS-MPs alone had no remarkable effects on the two biomarkers at the selected concentrations. However, PS-MPs combined with other pollutants significantly affected the two biomarkers in most situations. For EROD activity, PS + metal ions (except Zn2+ at 1000 μg/L), PS + BFRs (except decabromodiphenyl oxide (BDE-209)) or PS+ trimethoprim (TMP) significantly inhibited activity values, whereas PS+ 4-acetaminophen (AMP) induced EROD activity. For GST, PS together with most tested pollutants (except PS+ ibuprofen (IBF)) greatly decreased the activities. Accordingly, future research should focus on combined toxicity of mixtures to set more reasonable environmental safety evaluation standards.</div>","PeriodicalId":54423,"journal":{"name":"Toxicology in Vitro","volume":"100 ","pages":"Article 105915"},"PeriodicalIF":2.6000,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Toxicology in Vitro","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0887233324001450","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"TOXICOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Microplastic (MP) pollution is a potential threat to marine organisms. In vitro toxicity of MPs and other pollutants, such as pharmaceutically active compounds (PhACs) and brominated flame retardants (BFRs), has been understudied. This study aimed to investigate the effects of polystyrene microplastics (PS-MPs) with different particle sizes on two biomarkers: ethoxyresorufin-O-deethylase (EROD) and glutathione S-transferase (GST) in tilapia liver homogenates. The study also examined the combined effects of PS-MPs with various environmental contaminants, including three metal ions (Cu²⁺, Zn²⁺, Pb²⁺), three BFRs, and six PhACs. PS-MPs alone had no remarkable effects on the two biomarkers at the selected concentrations. However, PS-MPs combined with other pollutants significantly affected the two biomarkers in most situations. For EROD activity, PS + metal ions (except Zn²⁺ at 1000 μg/L), PS + BFRs (except decabromodiphenyl oxide (BDE-209)) or PS+ trimethoprim (TMP) significantly inhibited activity values, whereas PS+ 4-acetaminophen (AMP) induced EROD activity. For GST, PS together with most tested pollutants (except PS+ ibuprofen (IBF)) greatly decreased the activities. Accordingly, future research should focus on combined toxicity of mixtures to set more reasonable environmental safety evaluation standards.

查看原文本刊更多论文

聚苯乙烯微塑料和环境污染物对罗非鱼乙氧苏鲁素-O-脱乙基酶和谷胱甘肽 S-转移酶活性的体外影响。

微塑料（MP）污染是对海洋生物的潜在威胁。人们对微塑料和其他污染物（如药物活性化合物和溴化阻燃剂）的体外毒性研究不足。本研究旨在调查不同粒径的聚苯乙烯微塑料（PS-MPs）对罗非鱼肝脏匀浆中乙氧基树脂素-O-脱乙基酶（EROD）和谷胱甘肽 S-转移酶（GST）这两种生物标志物的影响。该研究还考察了 PS-MPs 与各种环境污染物（包括三种金属离子（Cu2+、Zn2+、Pb2+）、三种溴化阻燃剂和六种 PhACs）的综合效应。在选定的浓度下，单独使用 PS-MPs 对这两种生物标志物没有显著影响。不过，在大多数情况下，PS-MPs 与其他污染物结合会对这两种生物标志物产生重大影响。就 EROD 活性而言，PS + 金属离子（1000 微克/升的 Zn2+ 除外）、PS +溴化阻燃剂（十溴二苯醚（BDE-209）除外）或 PS + 三甲氧苄啶（TMP）会明显抑制活性值，而 PS + 4-乙酰氨基酚（AMP）则会诱导 EROD 活性。对于 GST 而言，PS 与大多数测试污染物（PS+ 布洛芬（IBF）除外）一起使用会大大降低其活性。因此，今后的研究应侧重于混合物的综合毒性，以制定更合理的环境安全评价标准。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Toxicology in Vitro 医学-毒理学

CiteScore

6.50

自引率

3.10%

发文量

181

审稿时长

65 days

期刊介绍： Toxicology in Vitro publishes original research papers and reviews on the application and use of in vitro systems for assessing or predicting the toxic effects of chemicals and elucidating their mechanisms of action. These in vitro techniques include utilizing cell or tissue cultures, isolated cells, tissue slices, subcellular fractions, transgenic cell cultures, and cells from transgenic organisms, as well as in silico modelling. The Journal will focus on investigations that involve the development and validation of new in vitro methods, e.g. for prediction of toxic effects based on traditional and in silico modelling; on the use of methods in high-throughput toxicology and pharmacology; elucidation of mechanisms of toxic action; the application of genomics, transcriptomics and proteomics in toxicology, as well as on comparative studies that characterise the relationship between in vitro and in vivo findings. The Journal strongly encourages the submission of manuscripts that focus on the development of in vitro methods, their practical applications and regulatory use (e.g. in the areas of food components cosmetics, pharmaceuticals, pesticides, and industrial chemicals). Toxicology in Vitro discourages papers that record reporting on toxicological effects from materials, such as plant extracts or herbal medicines, that have not been chemically characterized.

文献相关原料

公司名称	产品信息	采购帮参考价格
百灵威	erythromycin	￥23.00~￥9017.00