Effect of β-lactam antibiotics on the gut microbiota of term neonates.

IF 4.6 2区 医学 Q1 MICROBIOLOGY
Hongdan Gu, Enfu Tao, Yijia Fan, Gao Long, Xinyi Jia, Tianming Yuan, Lihua Chen, Xiaoli Shu, Wei Zheng, Mizu Jiang
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引用次数: 0

Abstract

β-Lactam antibiotics are a class of antibiotics commonly used to treat bacterial infections. However, the effects of β-lactam antibiotics on term neonatal intestinal flora have not been fully elucidated. Hospitalized full-term newborns receiving β-lactam antibiotics formed the antibiotic group (n = 67), while those without antibiotic treatment comprised the non-antibiotic group (n = 47). A healthy group included healthy full-term newborns (n = 16). Stool samples were collected for 16 S rDNA sequencing to analyze gut microbiota variations. Further investigation was carried out within the β-lactam antibiotic group, exploring the effects of antibiotic use on the newborns' gut microbiota in relation to the duration and type of antibiotic administration, delivery method, and feeding practices. The antibiotic group exhibited significant difference of microbial community composition compared to the other groups. Genera like Klebsiella, Enterococcus, Streptococcus, Alistipes, and Aeromonas were enriched, while Escherichia-Shigella, Clostridium sensu stricto 1, Bifidobacterium, and Parabacteroides were reduced. Klebsiella negatively correlated with Escherichia-Shigella, positively with Enterobacter, while Escherichia-Shigella negatively correlated with Enterococcus and Streptococcus. Regardless of neonatal age, β-lactam antibiotics induced an elevated abundance of Klebsiella and Enterococcus. The impact on gut microbiota varied with the duration and type of antibiotic (cefotaxime or ampicillin/sulbactam). Compared to vaginal delivery, cesarean delivery after β-lactam treatment heightened the abundance of Klebsiella, Enterobacteriaceae_Unclassified, Lactobacillales_Unclassified, and Pectobacterium. Feeding patterns minimally influenced β-lactam-induced alterations. In conclusion, β-lactam antibiotic treatment for neonatal pneumonia and sepsis markedly disrupted intestinal microbiota, favoring Klebsiella, Enterococcus, Streptococcus, Alistipes, and Aeromonas. The impact of β-lactam varied by duration, type, and delivery method, emphasizing heightened disruptions post-cesarean delivery.

β-内酰胺类抗生素对足月儿肠道微生物群的影响。
β-内酰胺类抗生素是一类常用于治疗细菌感染的抗生素。然而,β-内酰胺类抗生素对足月儿肠道菌群的影响尚未完全阐明。接受β-内酰胺类抗生素治疗的住院足月新生儿组成抗生素组(n = 67),未接受抗生素治疗的新生儿组成非抗生素组(n = 47)。健康组包括健康的足月新生儿(n = 16)。采集粪便样本进行 16 S rDNA 测序,以分析肠道微生物群的变化。在β-内酰胺类抗生素组中开展了进一步调查,探讨抗生素的使用对新生儿肠道微生物群的影响与抗生素用药时间、类型、分娩方式和喂养方式的关系。与其他组相比,抗生素组的微生物群落组成有明显差异。克雷伯菌属、肠球菌属、链球菌属、阿利斯蒂普斯菌属和气单胞菌属富集,而志贺氏菌属、严格梭状芽胞杆菌 1 型、双歧杆菌属和副杆菌属减少。克雷伯氏菌与埃希氏-志贺氏菌呈负相关,与肠杆菌呈正相关,而埃希氏-志贺氏菌与肠球菌和链球菌呈负相关。无论新生儿的年龄如何,β-内酰胺类抗生素都会引起克雷伯氏菌和肠球菌数量的增加。对肠道微生物群的影响因抗生素的持续时间和类型(头孢他啶或氨苄西林/舒巴坦)而异。与阴道分娩相比,β-内酰胺类药物治疗后的剖宫产增加了克雷伯氏菌、未分类肠杆菌、未分类乳杆菌和果胶杆菌的数量。饲喂模式对β-内酰胺引起的变化影响很小。总之,β-内酰胺类抗生素治疗新生儿肺炎和败血症明显破坏了肠道微生物群,有利于克雷伯氏菌、肠球菌、链球菌、阿利斯蒂普斯菌和气单胞菌。β-内酰胺的影响因持续时间、类型和分娩方式的不同而不同,尤其是剖腹产后的影响更大。
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来源期刊
CiteScore
8.60
自引率
0.00%
发文量
49
审稿时长
>12 weeks
期刊介绍: Annals of Clinical Microbiology and Antimicrobials considers good quality, novel and international research of more than regional relevance. Research must include epidemiological and/or clinical information about isolates, and the journal covers the clinical microbiology of bacteria, viruses and fungi, as well as antimicrobial treatment of infectious diseases. Annals of Clinical Microbiology and Antimicrobials is an open access, peer-reviewed journal focusing on information concerning clinical microbiology, infectious diseases and antimicrobials. The management of infectious disease is dependent on correct diagnosis and appropriate antimicrobial treatment, and with this in mind, the journal aims to improve the communication between laboratory and clinical science in the field of clinical microbiology and antimicrobial treatment. Furthermore, the journal has no restrictions on space or access; this ensures that the journal can reach the widest possible audience.
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