A hybrid genome assembly of the endangered aye-aye (Daubentonia madagascariensis).

IF 2.1 3区生物学 Q3 GENETICS & HEREDITY

G3: Genes|Genomes|Genetics Pub Date : 2024-10-07 DOI:10.1093/g3journal/jkae185

Cyril J Versoza, Susanne P Pfeifer

引用次数: 0

Abstract

The aye-aye (Daubentonia madagascariensis) is the only extant member of the Daubentoniidae primate family. Although several reference genomes exist for this endangered strepsirrhine primate, the predominant usage of short-read sequencing has resulted in limited assembly contiguity and completeness, and no protein-coding gene annotations have yet been released. Here, we present a novel, fully annotated, chromosome-level hybrid de novo assembly for the species based on a combination of Oxford Nanopore Technologies long reads and Illumina short reads and scaffolded using genome-wide chromatin interaction data-a community resource that will improve future conservation efforts as well as primate comparative analyses.

查看原文本刊更多论文

濒临灭绝的非洲羚（Daubentonia madagascariensis）的混合基因组组装。

羚牛（Daubentonia madagascariensis）是羚牛科灵长类动物中唯一现存的成员。虽然这种濒危的链臀目灵长类动物有几个参考基因组，但由于主要使用短线程测序，其组装的连续性和完整性有限，而且尚未发布蛋白编码基因注释。在此，我们基于牛津纳米孔技术公司（Oxford Nanopore Technologies）的长线程测序和 Illumina 的短线程测序，并利用全基因组染色质相互作用数据构建支架，为该物种提供了一个新颖的、注释完整的染色体级混合从头装配--这一社区资源将改善未来的保护工作以及灵长类动物的比较分析。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

G3: Genes|Genomes|Genetics GENETICS & HEREDITY-

CiteScore

5.10

自引率

3.80%

发文量

305

审稿时长

3-8 weeks

期刊介绍： G3: Genes, Genomes, Genetics provides a forum for the publication of high‐quality foundational research, particularly research that generates useful genetic and genomic information such as genome maps, single gene studies, genome‐wide association and QTL studies, as well as genome reports, mutant screens, and advances in methods and technology. The Editorial Board of G3 believes that rapid dissemination of these data is the necessary foundation for analysis that leads to mechanistic insights. G3, published by the Genetics Society of America, meets the critical and growing need of the genetics community for rapid review and publication of important results in all areas of genetics. G3 offers the opportunity to publish the puzzling finding or to present unpublished results that may not have been submitted for review and publication due to a perceived lack of a potential high-impact finding. G3 has earned the DOAJ Seal, which is a mark of certification for open access journals, awarded by DOAJ to journals that achieve a high level of openness, adhere to Best Practice and high publishing standards.