Exploring the In Vitro and In Vivo Therapeutic Efficacy of Juniperus oxycedrus Cade Oil: Antioxidant, Anti-inflammatory, and Anti-asthmatic Effects in an Allergic Asthma Model.

IF 1.5 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Meryem Ahmida, Mustapha Hichem Zadam, Nesrine Djaber, Taha Khaldi, Chawki Bensouici, Latifa Khattabi, Hichem Amara, Moncef Zaafour, Amel Boumendjel, Mahfoud Messarah, Mahieddine Boumendjel
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Abstract

This investigation aimed to explore the antioxidant, anti-inflammatory effects of Cade oil and its efficacy within a Wistar allergic asthma model. The antioxidant activity was assessed through various in vitro tests using chain-breaking antioxidant effects (radical scavenging and reducing abilities assays).  In vivo experiments involved Wistar rats categorized into four groups: negative control group, Ovalbumin-sensitised/challenged group, Cade oil-treated group, and Ovalbumin-sensitised/challenged Cade oil-treated group. These experiments aimed to evaluate oxidative stress parameters in the lungs and erythrocytes. The results indicated that the Cade oil exhibited significant antioxidant capabilities, evidenced by its radical scavenging activity against DPPH, ABTS, and Galvinoxyl radicals, with IC50 values ranging from 21.92 to 24.44 µg/mL. Besides, the reducing abilities methods showed A0,5 value ranging from 11.51 to 30.40  µg/mL for reducing power, Cupric ion reducing antioxidant capacity, and O-phenanthroline assays. Additionally, the IC50 value for β-carotene scavenging was found to be (8.2 ± 0.25 µg/ml). Analysis revealed high levels of polyphenols and flavonoids in Cade oil, indicating rich polyphenol (275.21 ± 3.14 mg GAE/g DW) and flavonoid (28.23 ± 1.91 µg QE/mg) content. In vivo findings highlighted Cade oil's efficacy in reducing inflammatory cell recruitment, enhancing antioxidant status, reducing lipid peroxidation, and improving histopathological alterations within the allergic asthma model. These results demonstrated that Cade oil has a potent antioxidant, anti-inflammatory, and anti-asthmatic properties, suggesting its potential therapeutic application in asthma treatment.

探索桧油的体外和体内疗效:过敏性哮喘模型中的抗氧化、抗炎和抗哮喘效果。
这项研究旨在探索卡德油的抗氧化、抗炎作用及其在 Wistar 过敏性哮喘模型中的功效。抗氧化活性是通过各种体外测试,使用断链抗氧化效应(自由基清除和还原能力测定)进行评估的。 体内实验将 Wistar 大鼠分为四组:阴性对照组、卵清蛋白致敏/哮喘组、Cade 油处理组和卵清蛋白致敏/哮喘 Cade 油处理组。这些实验旨在评估肺和红细胞中的氧化应激参数。结果表明,卡德油具有显著的抗氧化能力,其对 DPPH、ABTS 和 Galvinoxyl 自由基的清除活性证明了这一点,IC50 值介于 21.92 至 24.44 µg/mL 之间。此外,还原能力法、铜离子还原抗氧化能力法和 O-菲罗啉法的 A0,5 值为 11.51 至 30.40 µg/mL 不等。此外,β-胡萝卜素清除的 IC50 值为(8.2 ± 0.25 µg/ml)。分析表明,凯德油中的多酚类和类黄酮含量较高,表明其含有丰富的多酚类(275.21 ± 3.14 mg GAE/g DW)和类黄酮(28.23 ± 1.91 µg QE/mg)。体内研究结果表明,在过敏性哮喘模型中,凯德油具有减少炎症细胞募集、提高抗氧化状态、减少脂质过氧化和改善组织病理学改变的功效。这些结果表明,卡德油具有强大的抗氧化、抗炎和抗哮喘特性,这表明它在哮喘治疗中具有潜在的治疗用途。
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来源期刊
Cellular and molecular biology
Cellular and molecular biology 生物-生化与分子生物学
CiteScore
1.60
自引率
12.50%
发文量
331
期刊介绍: Cellular and Molecular Biology publishes original articles, reviews, short communications, methods, meta-analysis notes, letters to editor and comments in the interdisciplinary science of Cellular and Molecular Biology linking and integrating molecular biology, biophysics, biochemistry, enzymology, physiology and biotechnology in a dynamic cell and tissue biology environment, applied to human, animals, plants tissues as well to microbial and viral cells. The journal Cellular and Molecular Biology is therefore open to intense interdisciplinary exchanges in medical, dental, veterinary, pharmacological, botanical and biological researches for the demonstration of these multiple links.
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