Seed priming and subsequent inoculation of drought-tolerant plant growth-promoting rhizobacteria Bacillus tropicus KH90 improves rice (Oryza sativa L.) plant health under drought and non-drought conditions

IF 1.6 4区农林科学 Q2 AGRONOMY

Cereal Research Communications Pub Date : 2024-08-02 DOI:10.1007/s42976-024-00559-7

Abu Barkat Md Gulzar, Pranab Behari Mazumder

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Abstract

Drought stress adversely affects productivity by altering plant health. Plant growth-promoting rhizobacteria (PGPR) can boost plant health and induce plant tolerance against various unfavorable conditions. This study aimed to screen drought-tolerant rhizobacteria with potent multi-PGP traits from previously isolated strains and evaluate the isolates’ efficacy on the enhancement of rice seed growth parameters under drought and non-drought conditions. Five isolates (KK21, MK66, BH73, BH74, and KH90) were found to be drought-tolerant with multi-PGP traits under both drought and non-drought conditions. The isolates also showed efficient oxidative stress mitigation features like proline accumulation, SA-production, and antioxidant (PPO, PO, and CAT) activities. The FTIR research revealed the presence of several functional groups in KH90-produced EPS under stressed and non-stressed circumstances. Using 16S rRNA gene sequencing, the drought-tolerant isolates were identified as Bacillus cereus KK21, Lysinibacillus fusiformis MK66, Bacillus luti BH73, Bacillus aerius BH74, and Bacillus tropicus KH90. Primed seeds performed better than unprimed seeds in terms of germination percentage, seedling height, and vigor index when grown in both drought and non-drought conditions. However, further plant inoculation with KH90 increased leaf relative water, photosynthetic pigments, and proline content and decreased electrolyte leakage and malondialdehyde contents in rice leaves under both drought and non-drought conditions. The results showed that drought-tolerant Bacillus tropicus KH90 with multi-PGP-traits is able to improve rice plant health in both normal and drought-stressed conditions by reducing the physiological damage caused by the stress. This gave credence to the idea that this strain may be used to manage drought stress.

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种子处理和随后接种耐旱植物生长促进根瘤菌枯草芽孢杆菌 KH90 可改善水稻（Oryza sativa L. ）在干旱和非干旱条件下的植株健康状况

干旱胁迫会改变植物的健康状况，从而对生产力产生不利影响。植物生长促进根瘤菌（PGPR）可以促进植物健康，诱导植物耐受各种不利条件。本研究旨在从以前分离的菌株中筛选出具有强效多PGP性状的耐旱根瘤菌，并评估这些分离株在干旱和非干旱条件下对提高水稻种子生长参数的功效。结果发现，五个分离株（KK21、MK66、BH73、BH74 和 KH90）在干旱和非干旱条件下均具有耐旱性和多重PGP 性状。这些分离物还表现出有效的氧化胁迫缓解特征，如脯氨酸积累、SA 生产和抗氧化（PPO、PO 和 CAT）活性。傅立叶变换红外光谱研究显示，在胁迫和非胁迫条件下，KH90 产生的 EPS 中存在多个功能基团。通过 16S rRNA 基因测序，耐旱分离物被鉴定为蜡样芽孢杆菌 KK21、镰刀形溶血芽孢杆菌 MK66、卢蒂芽孢杆菌 BH73、枯草芽孢杆菌 BH74 和滋养芽孢杆菌 KH90。在干旱和非干旱条件下生长时，经过预处理的种子在发芽率、苗高和活力指数方面均优于未经过预处理的种子。然而，在干旱和非干旱条件下，进一步接种 KH90 能增加水稻叶片的相对水分、光合色素和脯氨酸含量，减少电解质渗漏和丙二醛含量。结果表明，具有多种PGP性状的耐旱芽孢杆菌 KH90 能够通过减少胁迫造成的生理损伤，改善水稻植株在正常和干旱胁迫条件下的健康状况。这使人们相信该菌株可用于管理干旱胁迫。

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来源期刊

Cereal Research Communications AGRONOMY-

CiteScore

3.40

自引率

6.20%

发文量

审稿时长

6-12 weeks

期刊介绍： This journal publishes original papers presenting new scientific results on breeding, genetics, physiology, pathology and production of primarily wheat, rye, barley, oats and maize.