Evaluation of the antiproliferative and oxidative effects of HEMA based polymeric cryogels including N-vinyl formamide on the model eukaryotic yeast Saccharomyces cerevisiae

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-08-02 DOI:10.1007/s11756-024-01759-6

Ayşenur Güler, Koray Şarkaya, Berna Kavakcıoğlu Yardımcı

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Abstract

Here, the effects of previously characterized poly(HEMA-N-vinyl formamide) (PHEMA-NVF) cryogels on viability, surface morphology, total antioxidant-oxidant status, antioxidant enzyme activities, and biochemical makeup of Saccharomyces cerevisiae were investigated for the first time. The cells' viability from the PHEMA-NVF cryogels exhibited a significant drop of 23.96 ± 1.44% compared to the plain PHEMA cryogel, which served as the control group without NVF. Additionally, this cryogel caused blebbing and holes on the cell surface. Despite the gradual increase in TOS levels with increasing NVF amounts, only the cryogel containing 3.12 mmol NVF showed a statistically significant difference from the control. The gradual increases in TAS levels in response to cellular stress were recorded depending on the increase in NVF amounts up to 3.12 mmol. While the enzymatic antioxidant system was generally induced in cells released from the cryogel prepared with relatively low levels of NVF, these activities dramatically decreased at higher amounts of the comonomer. Finally, FTIR analysis revealed that the highest concentration of NVF caused protein degradation and increased the relative concentration of lipid and polysaccharide molecules. Therefore, we can suggest that this comonomer induces oxidative stress, which the cell cannot tolerate beyond a certain threshold.

Graphical Abstract

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评估基于 HEMA（包括 N-乙烯基甲酰胺）的聚合物低温凝胶对模式真核酵母酿酒酵母的抗增殖和氧化作用

本文首次研究了先前表征的聚（HEMA-N-乙烯基甲酰胺）（PHEMA-NVF）低温凝胶对酿酒酵母的活力、表面形态、总抗氧化状态、抗氧化酶活性和生化组成的影响。与不含 NVF 的普通 PHEMA 低温凝胶对照组相比，PHEMA-NVF 低温凝胶的细胞活力显著下降了 23.96 ± 1.44%。此外，这种冷凝凝胶还导致细胞表面出现出血和孔洞。尽管随着 NVF 含量的增加，TOS 含量也逐渐增加，但只有含有 3.12 mmol NVF 的冷凝胶与对照组相比有显著的统计学差异。随着 NVF 含量的增加，细胞应激反应中的 TAS 水平逐渐增加，最高可达 3.12 毫摩尔。虽然用较低浓度的 NVF 制备的低温凝胶释放出的细胞一般都能诱导酶抗氧化系统，但当共聚物含量较高时，这些活性就会急剧下降。最后，傅立叶变换红外分析显示，最高浓度的 NVF 会导致蛋白质降解，并增加脂质和多糖分子的相对浓度。因此，我们可以认为，这种共聚单体会诱发氧化应激，细胞无法承受超过一定阈值的氧化应激。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.