An integrated network pharmacology and molecular docking approach to reveal the role of Arctigenin against Cutibacterium acnes-induced skin inflammation by targeting the CYP19A1

IF 3.2 4区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Xiaoyan Lu, Yanzhong Han, Yongkang Zhang, Rui Li, Jiaoyan Xu, Jian Yang, Jingchun Yao, Zhihai Lv
{"title":"An integrated network pharmacology and molecular docking approach to reveal the role of Arctigenin against Cutibacterium acnes-induced skin inflammation by targeting the CYP19A1","authors":"Xiaoyan Lu,&nbsp;Yanzhong Han,&nbsp;Yongkang Zhang,&nbsp;Rui Li,&nbsp;Jiaoyan Xu,&nbsp;Jian Yang,&nbsp;Jingchun Yao,&nbsp;Zhihai Lv","doi":"10.1111/cbdd.14598","DOIUrl":null,"url":null,"abstract":"<p>Acne caused by inflammation of hair follicles and sebaceous glands is a common chronic skin disease. Arctigenin (ATG) is an extract of Arctium lappa L., which has significant anti-inflammatory effects. However, the effect and mechanism of ATG in cutaneous inflammation mediated by <i>Cutibacterium acnes</i> (<i>C</i>. <i>acnes</i>) has not been fully evaluated. The purpose of this study was to explore the effect and potential mechanism of ATG in the treatment of acne through network pharmacology and experimental confirmation. An acne model was established by injected live <i>C. acnes</i> into living mice and treated with ATG. Our data showed that ATG effectively improved acne induced by live <i>C. acnes</i>, which was confirmed by determining ear swelling rate, estradiol concentration and hematoxylin and eosin (H&amp;E) staining. In addition, ATG inhibited the NLRP3 inflammasome signaling pathway in mice ear tissues and reduced the secretion of pro-inflammatory cytokines IL-1β to relieve inflammation. The results of network pharmacology and molecular docking confirmed that ATG can regulate 17β-Estradiol (E2) levels through targeted to CYP19A1, and finally inhibited skin inflammation. Taken together, our results confirmed that ATG regulated E2 secretion by targeting CYP19A1, thereby inhibiting the NLRP3 inflammasome signaling pathway and improving inflammation levels in acne mice. This study provides a basis for the feasibility of ATG in treating acne in clinical practice.</p>","PeriodicalId":143,"journal":{"name":"Chemical Biology & Drug Design","volume":"104 2","pages":""},"PeriodicalIF":3.2000,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chemical Biology & Drug Design","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/cbdd.14598","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Acne caused by inflammation of hair follicles and sebaceous glands is a common chronic skin disease. Arctigenin (ATG) is an extract of Arctium lappa L., which has significant anti-inflammatory effects. However, the effect and mechanism of ATG in cutaneous inflammation mediated by Cutibacterium acnes (C. acnes) has not been fully evaluated. The purpose of this study was to explore the effect and potential mechanism of ATG in the treatment of acne through network pharmacology and experimental confirmation. An acne model was established by injected live C. acnes into living mice and treated with ATG. Our data showed that ATG effectively improved acne induced by live C. acnes, which was confirmed by determining ear swelling rate, estradiol concentration and hematoxylin and eosin (H&E) staining. In addition, ATG inhibited the NLRP3 inflammasome signaling pathway in mice ear tissues and reduced the secretion of pro-inflammatory cytokines IL-1β to relieve inflammation. The results of network pharmacology and molecular docking confirmed that ATG can regulate 17β-Estradiol (E2) levels through targeted to CYP19A1, and finally inhibited skin inflammation. Taken together, our results confirmed that ATG regulated E2 secretion by targeting CYP19A1, thereby inhibiting the NLRP3 inflammasome signaling pathway and improving inflammation levels in acne mice. This study provides a basis for the feasibility of ATG in treating acne in clinical practice.

Abstract Image

Abstract Image

通过整合网络药理学和分子对接方法,揭示 Arctigenin 通过靶向 CYP19A1 对抗痤疮棒状杆菌诱发的皮肤炎症的作用。
由毛囊和皮脂腺炎症引起的痤疮是一种常见的慢性皮肤病。牛蒡苷(ATG)是一种牛蒡提取物,具有显著的抗炎作用。然而,ATG 在痤疮棒状杆菌(C. acnes)介导的皮肤炎症中的作用和机制尚未得到充分评估。本研究旨在通过网络药理学和实验证实,探索 ATG 治疗痤疮的效果和潜在机制。通过将活的痤疮丙酸杆菌注射到活体小鼠体内并用 ATG 治疗,建立了痤疮模型。我们的数据显示,ATG能有效改善活痤疮丙酸杆菌诱导的痤疮,这一点通过测定耳肿胀率、雌二醇浓度和苏木精及伊红(H&E)染色得到了证实。此外,ATG还能抑制小鼠耳组织中的NLRP3炎性体信号通路,减少促炎细胞因子IL-1β的分泌,从而缓解炎症。网络药理学和分子对接的结果证实,ATG能通过靶向CYP19A1调节17β-雌二醇(E2)水平,并最终抑制皮肤炎症。综上所述,我们的研究结果证实,ATG通过靶向CYP19A1调节E2分泌,从而抑制NLRP3炎症信号通路,改善痤疮小鼠的炎症水平。这项研究为ATG在临床上治疗痤疮的可行性提供了依据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Chemical Biology & Drug Design
Chemical Biology & Drug Design 医学-生化与分子生物学
CiteScore
5.10
自引率
3.30%
发文量
164
审稿时长
4.4 months
期刊介绍: Chemical Biology & Drug Design is a peer-reviewed scientific journal that is dedicated to the advancement of innovative science, technology and medicine with a focus on the multidisciplinary fields of chemical biology and drug design. It is the aim of Chemical Biology & Drug Design to capture significant research and drug discovery that highlights new concepts, insight and new findings within the scope of chemical biology and drug design.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信