Comprehensive DNA metabarcoding-based detection of non-indigenous invertebrates in recreational marinas through a multi-substrate approach

IF 3 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

Marine environmental research Pub Date : 2024-07-30 DOI:10.1016/j.marenvres.2024.106660

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引用次数: 0

Abstract

eDNA metabarcoding has been increasingly employed in the monitoring of marine invertebrate non-indigenous species (NIS), in particular using filtered seawater. However, comprehensive detection of all NIS may require a diversity of sampling substrates. To assess the effectiveness of 5 sample types (hard and artificial substrates, water, zooplankton) on the recovery of invertebrates’ diversity, two marinas were monitored over three time points, using COI and 18S rRNA genes as DNA metabarcoding markers. We detected a total of 628 species and 23 NIS, with only up to 9% species and 17% of NIS detected by all sample types. Hard and artificial substrates were similar to each other but displayed the most significant difference in invertebrate recovery when compared to water eDNA and zooplankton. Five NIS are potential first records for Portugal. No NIS were detected in all sample types and seasons, highlighting the need for varied sampling approaches, and consideration of temporal variation for comprehensive marine NIS surveillance.

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查看原文本刊更多论文

通过多基质方法全面检测休闲游艇停泊区的非本地无脊椎动物 DNA 代谢编码。

在监测海洋无脊椎动物非土著物种（NIS）时，越来越多地采用 eDNA 代谢条形码，特别是使用过滤海水。然而，要全面检测所有 NIS，可能需要多种采样基质。为了评估 5 种样本类型（硬基质和人工基质、水、浮游动物）对恢复无脊椎动物多样性的有效性，我们使用 COI 和 18S rRNA 基因作为 DNA 代谢编码标记，对两个游艇码头进行了三个时间点的监测。我们共检测到 628 个物种和 23 个 NIS，所有样本类型检测到的物种和 NIS 分别只有 9% 和 17%。硬基质和人工基质的无脊椎动物检出率相近，但与水中的 eDNA 和浮游动物相比，两者的检出率差异最大。五种 NIS 可能是葡萄牙的首次记录。在所有样本类型和季节中均未检测到 NIS，这突出表明需要采用不同的取样方法，并考虑时间变化，以进行全面的海洋 NIS 监测。

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来源期刊

Marine environmental research 环境科学-毒理学

CiteScore

5.90

自引率

3.00%

发文量

217

审稿时长

46 days

期刊介绍： Marine Environmental Research publishes original research papers on chemical, physical, and biological interactions in the oceans and coastal waters. The journal serves as a forum for new information on biology, chemistry, and toxicology and syntheses that advance understanding of marine environmental processes. Submission of multidisciplinary studies is encouraged. Studies that utilize experimental approaches to clarify the roles of anthropogenic and natural causes of changes in marine ecosystems are especially welcome, as are those studies that represent new developments of a theoretical or conceptual aspect of marine science. All papers published in this journal are reviewed by qualified peers prior to acceptance and publication. Examples of topics considered to be appropriate for the journal include, but are not limited to, the following: – The extent, persistence, and consequences of change and the recovery from such change in natural marine systems – The biochemical, physiological, and ecological consequences of contaminants to marine organisms and ecosystems – The biogeochemistry of naturally occurring and anthropogenic substances – Models that describe and predict the above processes – Monitoring studies, to the extent that their results provide new information on functional processes – Methodological papers describing improved quantitative techniques for the marine sciences.