Bioactive compounds and antioxidant activity in various parts of Morus alba L. Cv. ichinose: a comparative analysis

IF 1.9 4区物理与天体物理 Q2 BIOLOGY

Origins of Life and Evolution of Biospheres Pub Date : 2024-07-30 DOI:10.1007/s11084-024-09650-9

Lubna Altaf, Shabir Ahmad Wani, Peerzada Rashid Hussain, Prashant Suradkar, Mohmmad Farooq Baqual, Aabid Ahmad Bhat

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Abstract

Present investigation was undertaken to assess the composition of bioactive compounds within various parts of methanolic extracts from mulberry plants utilizing both High-Performance Liquid Chromatography (HPLC) and spectrophotometric methodologies. Furthermore, the antioxidant potential was evaluated through assays including 2,2-diphenyl-1-picrylhydrazyl (DPPH), Ferric Reducing Antioxidant Power (FRAP), β-carotene bleaching (BCBA), and Hydroxyl Radical Scavenging Activity (HRSA). This research incorporated the analysis of the fruit, root, shoot, and leaf of the mulberry (Morus alba L.) Cv. Ichinose. Findings from the investigation revealed that levels of total phenols (533.8 ± 15.6 mg/100 g) and flavonoids (232.3 ± 7.1 mg/100 g) were notably higher in fruit compared to root, while total ascorbic acid content (70.9 ± 3.1 mg/100 g) was significantly elevated in the leaf. The fruit exhibited a significantly higher concentration of total anthocyanins (142.5 ± 3.1 mg/100 g), whereas the leaf contained a substantial amount of total carotenoids (5.8 ± 0.3 mg/100 g). Predominant phenolic acids detected in mulberry included ferulic acid, m-coumaric acid, syringic acid, caffeic acid, chlorogenic acid, and gallic acid. Quercetin and isoquercetin were identified as the major flavonoids in mulberry fruits. In terms of anthocyanins, cyanidin-3-rutinoside (25.6 ± 2.3 mg/100 g) and cyanidin-3-glucoside (16.4 ± 2.1 mg/100 g) were the primary anthocyanins present in the fruit. The main carotenoid found in the leaf was beta-carotene, with levels reaching 2.1 ± 0.3 mg/100 g. Fruit displayed the highest antioxidant activity among all parts examined. Comparison of the EC50 values of fruit (EC50 = 174.2 ± 2.2 µg/mL), root (EC50 = 179.6 ± 2.3 µg/mL), and shoot (EC50 = 189.4 ± 2.1) suggested that these segments of the mulberry plant could potentially function as antioxidants and could exhibit a greater hydroxyl radical scavenging effect than standard antioxidants.

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Morus alba L. Cv. ichinose 不同部位的生物活性化合物和抗氧化活性：比较分析

本研究利用高效液相色谱法（HPLC）和分光光度法评估了桑树植物甲醇提取物各部分中生物活性化合物的组成。此外，还通过 2,2-二苯基-1-苦基肼（DPPH）、铁还原抗氧化力（FRAP）、β-胡萝卜素漂白（BCBA）和羟基自由基清除活性（HRSA）等检测方法评估了抗氧化潜力。这项研究分析了桑树（Morus alba L.) Cv.一之濑。调查结果显示，果实中的总酚含量（533.8 ± 15.6 mg/100 g）和类黄酮含量（232.3 ± 7.1 mg/100 g）明显高于根部，而叶片中的总抗坏血酸含量（70.9 ± 3.1 mg/100 g）显著高于根部。果实中的总花青素含量（142.5 ± 3.1 毫克/100 克）明显更高，而叶片中则含有大量的总类胡萝卜素（5.8 ± 0.3 毫克/100 克）。桑椹中检测到的主要酚酸包括阿魏酸、间香豆酸、丁香酸、咖啡酸、绿原酸和没食子酸。桑果中的主要黄酮类化合物是槲皮素和异槲皮素。在花青素方面，青花素-3-芸香糖苷（25.6 ± 2.3 毫克/100 克）和青花素-3-葡萄糖苷（16.4 ± 2.1 毫克/100 克）是桑果中的主要花青素。叶片中的主要类胡萝卜素是 beta-胡萝卜素，含量达到 2.1 ± 0.3 毫克/100 克。对果实（EC50 = 174.2 ± 2.2 µg/mL）、根（EC50 = 179.6 ± 2.3 µg/mL）和芽（EC50 = 189.4 ± 2.1）的 EC50 值进行比较后发现，桑树植物的这些部分可能具有抗氧化功能，并且比标准抗氧化剂具有更强的清除羟基自由基的效果。

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来源期刊

Origins of Life and Evolution of Biospheres 生物-生物学

CiteScore

3.20

自引率

15.00%

发文量

审稿时长

>12 weeks

期刊介绍： The subject of the origin and early evolution of life is an inseparable part of the general discipline of Astrobiology. The journal Origins of Life and Evolution of Biospheres places special importance on the interconnection as well as the interdisciplinary nature of these fields, as is reflected in its subject coverage. While any scientific study which contributes to our understanding of the origins, evolution and distribution of life in the Universe is suitable for inclusion in the journal, some examples of important areas of interest are: prebiotic chemistry and the nature of Earth''s early environment, self-replicating and self-organizing systems, the theory of the RNA world and of other possible precursor systems, and the problem of the origin of the genetic code. Early evolution of life - as revealed by such techniques as the elucidation of biochemical pathways, molecular phylogeny, the study of Precambrian sediments and fossils and of major innovations in microbial evolution - forms a second focus. As a larger and more general context for these areas, Astrobiology refers to the origin and evolution of life in a cosmic setting, and includes interstellar chemistry, planetary atmospheres and habitable zones, the organic chemistry of comets, meteorites, asteroids and other small bodies, biological adaptation to extreme environments, life detection and related areas. Experimental papers, theoretical articles and authorative literature reviews are all appropriate forms for submission to the journal. In the coming years, Astrobiology will play an even greater role in defining the journal''s coverage and keeping Origins of Life and Evolution of Biospheres well-placed in this growing interdisciplinary field.