A Study on the Role of miR-126 in the Repair Process after Spinal Cord Injury

IF 2.1 4区医学 Q4 CELL & TISSUE ENGINEERING

Current stem cell research & therapy Pub Date : 2024-07-30 DOI:10.2174/011574888x289224240723042538

Yaojun Suo, Chunfang Wang

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引用次数: 0

Abstract

Background: Spinal Cord Injury (SCI) results in motor, sensory, and autonomic dysfunctions and causes social and economic problems. Surgery, medication, and stem cell transplantation are therapeutic strategies for SCI. The use of endogenous neural stem cells seems preferable due to their lower immune responses. miR-126 serves as a promising microRNA for reducing inflammation after SCI. It can promote angiogenesis and proliferation of neural stem cells Objectives: This study aimed to observe changes in miR-126 expression after SCI in an animal mice model. Methods: A total of 42 healthy adult FVB mice were divided equally into 7 groups (6 SCI model versus 1 control). At different periods following SCI establishment in the model groups, Basso Mouse Scale score (BMS), histopathological changes, and expression levels of miR-126 were evaluated in the model groups compared to the control one. Results:: The BMS score increased to a certain extent as the time after spinal cord injury progressed. HE and Nissl staining showed that the acute period (1-7 days) after spinal cord injury was characterized by neuronal loss, whereas the chronic phase (21st day) was characterized by scar and cavity formation. Compared with the control group, the model group exhibited decreased expression of miR-126 during the acute phase (days 1-7 post-SCI). However, its expression increased by 21th day after SCI. Conclusion: Overexpressed miR-126 can contribute to reduced SCI-related damages, which may result in the promotion of the growth and proliferation of neural stem cells as well as the repair of motor function.

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关于 miR-126 在脊髓损伤后修复过程中的作用的研究

背景：脊髓损伤（SCI）会导致运动、感觉和自主神经功能障碍，造成社会和经济问题。手术、药物治疗和干细胞移植是脊髓损伤的治疗策略。使用内源性神经干细胞似乎更可取，因为它们的免疫反应较低。它能促进血管生成和神经干细胞的增殖：本研究旨在通过动物小鼠模型观察 SCI 后 miR-126 表达的变化。方法：将 42 只健康成年 FVB 小鼠平均分为 7 组（6 组 SCI 模型与 1 组对照）。在模型组建立 SCI 后的不同时期，评估模型组与对照组相比的巴索小鼠量表评分（BMS）、组织病理学变化和 miR-126 的表达水平。结果随着脊髓损伤时间的延长，BMS评分有一定程度的增加。HE 和 Nissl 染色显示，脊髓损伤后的急性期（1-7 天）以神经元丢失为特征，而慢性期（第 21 天）以瘢痕和空洞形成为特征。与对照组相比，模型组在急性期（脊髓损伤后第 1-7 天）的 miR-126 表达量减少。然而，在 SCI 后第 21 天，miR-126 的表达增加。结论过表达的 miR-126 可减少 SCI 相关损伤，从而促进神经干细胞的生长和增殖以及运动功能的修复。

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来源期刊

Current stem cell research & therapy CELL & TISSUE ENGINEERING-CELL BIOLOGY

CiteScore

4.20

自引率

3.70%

发文量

197

审稿时长

>12 weeks

期刊介绍： Current Stem Cell Research & Therapy publishes high quality frontier reviews, drug clinical trial studies and guest edited issues on all aspects of basic research on stem cells and their uses in clinical therapy. The journal is essential reading for all researchers and clinicians involved in stem cells research.