Electrochemiluminescence microscopy for the investigation of peptides interactions within planar lipid membranes

IF 3.3 3区化学 Q2 CHEMISTRY, PHYSICAL

Faraday Discussions Pub Date : 2024-07-31 DOI:10.1039/d4fd00137k

Kaoru Hiramoto, Kosuke Ino, Ibuki Takahashi, Ayumi Hirano-Iwata, Hitoshi Shiku

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Abstract

Understanding the interactions between lipid membranes and peptides is crucial for controlling bacterial and viral infections, and developing effective drugs. In this study, we proposed the use of electrochemiluminescence (ECL) microscopy in a solution of [Ru(bpy)₃]²⁺ and tri-n-propylamine to monitor alterations in the lipid membranes due to peptide action. A planar artificial lipid membrane served as a model platform, and its surface was observed using ECL microscopy during exposure to melittin, a representative membrane lytic peptide. Upon exposure to melittin, the light-emitting process of the [Ru(bpy)₃]²⁺/tri-n-propylamine system through the lipid membrane exhibited complex changes, suggesting that stepwise peptide actions can be monitored through the system. Furthermore, wide-field imaging with ECL microscopy provided an effective means of elucidating the membrane surface at the submicron level and revealing heterogeneous changes upon exposure to melittin. This complemented the spatiotemporal information that could not be obtained using conventional electrochemical measurements

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电化学发光显微镜用于研究平面脂膜中肽的相互作用

了解脂膜与肽之间的相互作用对于控制细菌和病毒感染以及开发有效药物至关重要。在本研究中，我们提出了在[Ru(bpy)3]2+和三正丙胺溶液中使用电化学发光（ECL）显微镜来监测多肽作用引起的脂膜变化。以平面人工脂膜为模型平台，使用 ECL 显微镜观察其表面在暴露于具有代表性的膜溶解肽 melittin 时的变化。暴露于美乐汀时，[Ru(py)3]2+/三正丙胺体系透过脂膜的发光过程表现出复杂的变化，表明可以通过该体系监测肽的分步作用。此外，利用 ECL 显微镜进行宽视场成像提供了一种有效的方法，可在亚微米水平上阐明膜表面的情况，并揭示暴露于美利汀后的异质性变化。这补充了传统电化学测量无法获得的时空信息

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来源期刊

Faraday Discussions 化学-物理化学

自引率

0.00%

发文量

259

期刊介绍： Discussion summary and research papers from discussion meetings that focus on rapidly developing areas of physical chemistry and its interfaces